Background: The chick embryo is a classical model to study embryonic development. However, most researchers have not studied the effect of embryonic manipulation on chick hatchability. To determine the effect of egg orientation and type of sealing film on the hatchability of cultured embryos, and to determine the fate of adulte stem cells injected into the blastoderm. Materials and Methods: Win...

Human metastatic melanoma cells express a dedifferentiated, plastic phenotype, which may serve as a selective advantage, because melanoma cells invade various microenvironments. Over the last three decades, there has been an increased focus on the role of the tumor microenvironment in cancer progression, with the goal of reversing the metastatic phenotype. Here, using an embryonic chick model, ...

BACKGROUND Aluminium (Al) is known to have neurotoxic effects that can result in oxidative damage to a range of cellular biomolecules. These effects appear to be of significance in the developmental stages of the brain. We therefore investigated the oxidative and histopathological damage induced by Al during growth and development of the chick brain. MATERIAL/METHODS We used a chick embryonic...

Fertilized chicken eggs are suggested as an alternative to mammalian models. The chorioallantoic membrane (CAM) of the chick embryo is widely used for examination of angiogenesis, xenotransplants and for virus production. Unfortunately, it is mostly not taken into account, that the chick embryo's ability to experience pain starts to develop at day 7 of breeding. In our view, this model is only ...

The study aimed to investigate the effect of in ovo (IO) leptin injection on yolk sac concentration, embryo development, blood lipid metabolism, and biochemical parameters broiler chicks during early post-hatching period. Hatching eggs were weighed placed an incubator with 100 eggs/4 replications/group. groups were: non-injected group (C); 3 injected μl phosphate buffer solution (PBS); 0.5 (L0....

An assay capable of recovering individual viable rodent cells localized in various organs of the chick embryo is described. This assay is based on the differential sensitivity of chick and rodent cells to the cytotoxic drug ouabain. Utilizing this assay, the potential of the chick embryo as a model system for studying metastasis was examined. Several cell lines were characterized in three ways:...