نتایج جستجو برای: cell separation

تعداد نتایج: 1791062  

2010
C. H. Hsu C. C. Chen D. Irimia M. Toner

Obtaining pure cell subpopulations is often the first step for a wide range of applications. Here, we present a generic cell isolation strategy, the “buoyancy activated cell sorting (BACS)”, using glass microbubbles attached with targetspecific antibodies. The design of BACS was centered on the opposite movements of cells and microbubbles during mixing, ultimately improving the capture yield by...

El ż bieta Ograczyk Krzysztof Krawczyk Magdalena Kowalewicz-Kulbat Marcin W ł odarczyk Marek Fol,

Background: The immunomagnetic separation technique is the basis of monocyte isolation and further generation of monocyte-derived dendritic cells. Objective: To compare the efficiency of monocyte positive and negative separation, concentration of beads, and their impact on generated dendritic cells. Methods: Monocytes were obtained using monoclonal antibody-coated magnetic beads followed the Fi...

Journal: :Methods in molecular biology 2013
Mariana T Cerqueira Ana M Frias Rui L Reis Alexandra P Marques

Epidermal stem cells isolation struggle remains, mainly due to the yet essential requirement of well-defined approaches and markers. The herein proposed methodology integrates an assemblage of strategies to accomplish the enrichment of the interfollicular epidermal stem cells multipotent fraction and their subsequent separation from the remaining primary human keratinocytes culture. Those inclu...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه تبریز 1390

امروزه با توسعه روز افزون تکنولوژی mems و کاربردهای گسترده آن در مهندسی پزشکی می توان قطعاتی را طراحی نمود که قابل کاشت (implantable ) در داخل بدن باشد. اندازه گیری مقدار قند خون یکی از زمینه هایی می باشد که با توسعه تکنولوژی memsمورد توجه بوده است. در بیماران دیابتی نوع دوم، میزان قند خون بالا می باشد و از آنجایی که با افزایش قند خون احتمال بیهوشی نیز بالا می رود، لذا اندازه گیری قند خون بصورت...

Journal: :Methods in molecular medicine 2003
Geert Stangé Mark Van De Casteele Harry Heimberg

Journal: :Biochemical Society transactions 1992
P A Dyer

230 Introduction The diversity among cells of the body can be revealed in vitro by physical methods such as lightand electron-microscopy, size and density separations, active and physical adhesion to a solid phase and electrophoretic methods. Recently, much progress has been made in our understanding of the functions of cells by using antigedantibody interactions to perform negative and positiv...

2007
HENRI GRIZEL

-There is an ongoing need to isolate and purify ceIls in bivalve mollusc disease research. Many separation techniques differentiate cells solely by a single factor and usually require additives in the medium that may damage live cells. Centrifugai elutriation is a technique that separates particles by size and density simultaneously and can be conducted in physiological media without additives....

2001
R. Díaz S. Payen

Basic IC fabrication techniques are employed in this paper with the purpose of designing a microfluidic device with a 3D electrode arrangement to separate live and dead biological cells. This is done using the concept of dielectrophoresis, which describes the transnational motion of particles due to the application of a non-uniform electrical field. The simulations where carried out using the p...

Journal: :Journal of embryology and experimental morphology 1984
R Ransom R J Matela

Development in multicellular animals consists of a constant progression of cell division, differentiation and morphogenesis. Our understanding of the relationship between division and the acquisition of shape and form is not well understood, and this paper describes a computer representation of cell division processes with possible applications to the modelling of developmental events. This rep...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه رازی - پژوهشکده فنی و مهندسی 1388

asymmetric polyethersulfone (pes) microfiltration flat sheet membranes were composed by the phase inversion method (pim) and were used as supports. composite membranes were fabricated by coating silicone rubber as selective layer. effect of different concentrations of pes and pdms and different solvent such as nmp, dmf and dms effects as pes solvents and support thickness and different coagulat...

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