In this paper we describe a frequency-domain framework for source identification, separation and manipulation in stereo music recordings. Based on a simplified model of the stereo mix, we describe how a similarity measure between the Short-Time Fourier Transforms (STFT) of the input signals is used to identify time-frequency regions occupied by each source based on the panning coefficient assig...

Vector base amplitude panning (VBAP) is a generic method for virtual source positioning. It generalizes the pair-wise panning paradigm to triplet-wise panning paradigm, which can be used in three-dimensional loudspeaker setups. The VBAP implementation in MAX/MSP software presented in this paper is a powerful tool to produce spatialized computer music pieces that can be presented with di erent l...

In this paper, we analyze the visual discomfort caused by camera panning in 4K UHD contents. UHD is expected to provide a sense of realness to viewers, with four times more pixels than HD. However, UHD may yield undesirable artifacts, such as the stroboscopic effect from displaying frequency and visual fatigue due to moving objects. To investigate the visual discomfort in the presence of camera...

BACKGROUND The thorough understanding of the physiological and pathological processes mediated by extracellular vesicles (EVs) is challenged by purification methods which are cumbersome, not reproducible, or insufficient to yield homogeneous material. Chromatography based on both ion-exchange and immune-capture can represent an effective method to improve EV purification and successive analysis...

ABTAG is a camelid single-domain antibody (sdAb) that binds to bovine serum albumin (BSA) with low picomolar affinity. In surface plasmon resonance (SPR) analyses using BSA surfaces, bound ABTAG can be completely dissociated from the BSA surfaces at low pH, over multiple cycles, without any reduction in the capacity of the BSA surfaces to bind ABTAG. A moderate throughput, SPR-based, antibody s...

Germline precursors and intermediates of broadly neutralizing antibodies (bNAbs) are essential to the understanding of humoral response to HIV-1 infection and B-cell lineage vaccine design. Using a native-like gp140 trimer probe, we examined antibody libraries constructed from donor-17, the source of glycan-dependent PGT121-class bNAbs recognizing the N332 supersite on the HIV-1 envelope glycop...

Objective(s): As T-cell immunoglobulin and mucin domain 3 (TIM-3) is an immune regulatory molecule; its blocking or stimulating could alter the pattern of immune response towards a desired condition. Based on the unique features of nanobodies, we aimed to construct an anti-TIM-3 nanobody as an appropriate tool for manipulating immune responses for future therapeutic purposes. Materials and Meth...

Phage-displayed random peptide libraries, in which high affinity phage peptides are enriched by repetitive selection (panning) on target antibody, provide a unique tool for identifying antigen specificity. This paper describes a new panning method that enables selection of peptides in 1 day as compared to about 6 days required in traditional panning to identify virus-specific epitopes. The meth...