by immunizing mice with killed whole bacterial cells of brucella abortus s (99), a panel of six hybridomas producing monoclonal antibodies (mab) specific for the surface antigens of this bacterium were produced. elisa was used to screen the hybridoma supernatants. immunoblots of the cell extract indicated that three mab were specific for s-lps (ba-1, ba-2, ba-3) and three others were reactive w...

Hepatitis B immunoglobulin (HBIG) is an effective treatment for hepatitis B, including postexposure prophylaxis of HBV infection, prevention reinfection in liver transplant patients, and reducing sexual transmission. This study investigated the synthesis human IgG antibodies that specifically target HBsAg subtype adr CHL-YN cells, a newly established cell line grows faster than CHO-K1. To achie...

Kundu and Bertino (VLDB 2008) recently introduced the idea of structural signatures for trees which support public redaction of subtrees (by third-party distributors) while pertaining the integrity of the remaining parts. An example is given by signed XML documents of which parts should be sanitized before being published by a distributor not holding the signing key. Kundu and Bertino also prov...

Three hundred and ninety two faecal specimens from 70 separate outbreaks of suspected Clostridium perfringens food poisoning were examined by enzyme linked immunosorbent assay (ELISA), reversed passive latex agglutination (RPLA), and Vero cell assays for the presence of enterotoxin. Although the most time consuming method, ELISA was the most specific and reproducible. RPLA was slightly more sen...

herein, we presented the first report on bluetongue (bt) disease in 10 pregnant camels in a herd fromkerman province, iran. all sera samples were tested serologically (agid, c-elisa). we also used the razi-bk cell line, performed primary culture of ovine kidney and inoculated intravenously the embryonatedchicken eggs (ece) to culture and isolate the bt virus. efforts to culture and isolation of...

Commercial serologic assays for varicella-zoster virus (VZV), which enable reliable determination of VZV immune status and are amenable to automation, are needed. The present study compares the automated performance of the VZV whole-cell enzyme-linked immunosorbent assay (ELISA) Enzygnost anti-VZV/IgG, the Euroimmun anti-VZV ELISA (IgG) based on highly purified viral proteins, and the VZV glyco...

in this study a nested-pcr assay was optimized for detection of two bvdv biotype of nadl strain. a part of 5' non-coding region of virus, 249 bp in size, was amplified in rt-pcr. pcr product was cloned in a ptz57r/t vector and sequencing results confirmed the specificity of the test. internal primers were designed and a 155 bp dna fragment was amplified in nested-pcr. the sensitivity of rt...

introduction: the major histocompatibility complex (mhc) is a group of cell surface proteins that are essential for recognizing foreign molecules in human and other mammals. the physiologic function of mhc molecules is the presentation of peptides to t cells. in this study, we evaluated the purification of a class ii mhc molecule (hla-dr) from a human burkitt′s lymphoma cell line; daudi. materi...