Adenylate kinases (ADK) are ubiquitous enzymes that contribute to the homeostasis of adeninenucleotides in living cells. In this study, the cloning of a cDNA encoding an adenylate kinase from the filariaOnchocerca volvulus has been described. Using PCR technique, a 281 bp cDNA fragment encoding part ofan adenylate kinase was isolated from an O. volvulus cDNA library. Use of this fragment as a p...

Formosan subterranean termites, Coptotermes formosanus Shiraki, live socially in microbial-rich habitats. To understand the molecular mechanism by which termites combat pathogenic microbes, a full-length normalized cDNA library and four Suppression Subtractive Hybridization (SSH) libraries were constructed from termite workers infected with entomopathogenic fungi (Metarhizium anisopliae and Bea...

Integrative Annotation of 21,037 Human Genes Validated by Full-Length cDNA Clones Tadashi Imanishi , Takeshi Itoh , Yutaka Suzuki, Claire O’Donovan , Satoshi Fukuchi, et al. DOI: 10.1371/journal.pbio.0020162 1. The abbreviation FLJ was expanded incorrectly (as full-length long Japan) in the abbreviations list and in the fi rst paragraph of the Results/Discussion section. FLJ stands for full-len...

NBS-LRR-type disease resistance gene-like cDNA, induced by salicylic acid (SA) was cloned from rye Secalecereale L. (2n = 14RR) var. Petkus, which has rust genes such as Lr26, Sr31 andRr9. We designed primers based on the NBS region and performed PCR using Petkus genomic DNA a template. Next, we TA-cloned 532-bp fragment containing five homologous amino sequences in region. The SA-treated showe...

RNAseq is a relatively new tool for ecological genetics that offers researchers insight into changes in gene expression in response to a myriad of natural or experimental conditions. However, standard RNAseq methods (e.g., Illumina TruSeq® or NEBNext® ) can be cost prohibitive, especially when study designs require large sample sizes. Consequently, RNAseq is often underused as a method, or is a...

To our traditional library management, pursuing small and comprehensive library blindly or big and comprehensive one, which makes amounts of waste. In information society, people have varied requirements. To meet their requirements, library must positively develop more resources, positively organize the online Chinese information resources, provide effective online video service, greatly cultiv...

Hu sheep is one of the most important species in China; it is also listed as one of the 78 nationally protected domestic animals by the Chinese government in 2000. The construction of cDNA expression library of Hu sheep is of great significance for protecting individual genomes, generating transgenic sheep, and conducting clinical research using cDNA from Hu sheep. In this study, the total RNA ...

Here, we developed an improved method for constructing microdis sected cDNA libraries, based on strand-switching properties of reverse transcriptase, followed by PCR amplification with primers to mediate unidirectional insert cloning. Using RNA from microdissected ovarian carcinoma cells, we constructed a cDNA library consisting of 1.3 x 10' unidirectional recombinants with an average insert si...