The aim of this study was to characterize NK (CD56(+)CD3(-)) and NKT-like cell (CD56(+)CD3(+)) responses early after chikungunya infection. Expression profiling and functional analysis of T/NK/NKT-like cells were performed on samples from 56 acute and 31 convalescent chikungunya patients and 56 control individuals. The percentages of NK cells were high in both patient groups, whereas NKT-like c...

In an anti-glomerular basement membrane (GBM) glomerulonephritis (GN) model, GN-resistant Lewis rats naturally recover from early glomerular inflammation. Here we investigated recovery mechanisms for development of a potential immunotherapy for autoimmune GN. Our previous studies suggested that glomeruli-infiltrating leukocytes with a phenotype of CD8αα+CD11c+MHC-II+CD3- (GIL CD8αα+ cells) were...

BACKGROUND There is mounting evidence that perforin and granzymes are important mediators in the lung destruction seen in COPD. We investigated the characteristics of the three main perforin and granzyme containing peripheral cells, namely CD8+ T lymphocytes, natural killer (NK; CD56+CD3-) cells and NKT-like (CD56+CD3+) cells. METHODS Peripheral blood mononuclear cells (PBMCs) were isolated a...

Background: Monitoring of phenotypic characteristics of T-lymphocytes in peripheral blood is commonly performed to give the clinical parameters in the management of kidney transplant recipients.   Objective: To predict rejection in renal transplantation by immune parameters. Methods: 16 non-diabetic kidney transplant candidates (4 females and 12 males, age = 20-65 yr, first time transplant) wer...

background: monitoring of phenotypic characteristics of t-lymphocytes in peripheral blood is commonly performed to give the clinical parameters in the management of kidney transplant recipients.   objective: to predict rejection in renal transplantation by immune parameters. methods: 16 non-diabetic kidney transplant candidates (4 females and 12 males, age = 20-65 yr, first time transplant) wer...

Recently, we and others have reported tyrosine phosphorylation of phospholipase C-3'1 (PLC3,1) enzyme after CD3 activation ofT cells, and have proposed that PLC'yl mediates signal transduction through the T cell receptor (TCR/CD3). Here, using immunoblotting and immune complex PLC assays, we show that CD3 stimulation of Jurkat cells induces the association of PLC3,1 enzyme with CD3 complex. PLC...

Highly purified CD1-3-4-8- human thymocytes were obtained by panning techniques combined with cell depletion with antibody-coated magnetic beads. Most of these cells expressed cytoplasmic CD3 antigen, as assessed by mAbs known to react with the CD3 epsilon chain. After culture with low doses of PMA (0.5 ng/ml) and subsequent addition (at 24 h) of recombinant interleukin 2 (rIL-2; 100 U/ml) cell...

backgrounds: seminoma is frequently observed in human and canine testes especially in cryptorchids. canine and human seminomas are typically associated with leukocytic infiltration. objectives: we aimed to identify the type of lymphocytes that infiltrate to seminomas. methods: tumor infiltrating lymphocytes were evaluated by immunohistological techniques in 5 dogs with diffuse seminoma. routine...

immunophenotyping of lymphocytes is very essential for evaluation of immune system. due  to  the  effect  of  environmental  factors  and  ethnical diversity on  immune  system, establishment of an internal normal range of lymphocyte subsets is a necessity for each population.  the  aim  of  this  study  was  to  determine  the  normal  range  of  t  and  b lymphocytes, and nk cells in normal i...

The NKp46 receptor demonstrates a high degree of lineage specificity, being expressed almost exclusively in NK cells. Previous studies have demonstrated NKp46 expression by T cells, but NKp46+ CD3+ cells are rare and almost universally associated with NKp46 acquisition by T cells following stimulation. In this study we demonstrate the existence of a population of NKp46+ CD3+ cells resident in n...