Catharanthus roseus (L.) G. Don is a medicinal plant well known for producing antitumor drugs such as vinblastine and vincristine, which are classified as terpenoid indole alkaloids (TIAs). The TIA metabolic pathway in C. roseus has been extensively studied. However, the localization of TIA intermediates at the cellular level has not been demonstrated directly. In the present study, the metabol...

The subcellular localization of enzymes involved in indole alkaloid biosynthesis in leaves of Catharanthus roseus has been investigated. Tryptophan decarboxylase and strictosidine synthase which together produce strictosidine, the first indole alkaloid of this pathway, are both cytoplasmic enzymes. S-Adenosyl-l-methionine: 16-methoxy-2,3-dihydro-3-hydroxytabersonine-N-methyltransferase which ca...

Abstrak : Tapak dara (Catharanthus roseus)merupakan obat tradisional, dimana ekstrak baik dari daun ataupn bunga digunakan sebagai oleh masyarakat pedesaan. Perlu dianalisis sifat gel etanol yang dihasilkan yaitu penampakan etanol, viskositas, uji pembakaran, nilai kalor, panas dipindahkan, dan analisis warna api sehingga bisa kelihatan sediaan terbaik untuk menghasilkan etanol. Penelitian bers...

Periwinkle (Catharanthus roseus L.) (Family: Apocyanaceae) is a ornamental plants with great medicinal properties. Although it is represented by seven species, little work has been carried out on its genetic characterization due to non-availability of reliable molecular markers. Simple sequence repeats (SSRs) have been widely applied as molecular markers in genetic studies. With the rapid incre...

The majority of the Met synthesized in plants is utilized for methylations performed with S-adenosyl-L-Met. These reactions play a major role in the modification of a large variety of acceptor molecules, such as lipids, polysaccharides, nucleic acids, proteins, and secondary plant products (reviewed by Giovanelli, 1987). The hydrolysis of SAH to adenosine and L-homocysteine by SAH hydrolase is ...

Identification of molecular markers of monoterpenoid indole alkaloid (MIA) accumulation in cell-suspension cultures of Madagascar periwinkle (Catharanthus roseus (L.) G. Don) was performed by two-dimensional polyacrylamide gel electrophoresis. Comparison of the protein patterns from alkaloid-producing and non-producing cells showed the specific occurrence of a 28 kDa polypeptide restricted to c...

No study has been performed on identifying microRNAs (miRNAs) and their targets in the medicinal plant, Catharanthus roseus. In the present study, using the comparative genomics approach, we have predicted two potential C. roseus miRNAs. Furthermore, twelve potential mRNA targets were identified in C. roseus genome based on the characteristics that miRNAs exhibit perfect or nearly perfect compl...

Aspartame is an artificial sweetener in foods and beverages. Continuous use of aspartame will result oxidative stress the liver. So we need antioxidants from outside body such as periwinkle leaves.This study aims to determine effect leaves (Catharanthus roseus L) on aspartame-induced liver histology. This used 24 male mice divided into 4 groups, namely negative control (K-) without treatment; P...