نتایج جستجو برای: catharanthus roseus callus
تعداد نتایج: 6602 فیلتر نتایج به سال:
A cell suspension culture o f Catharanthus roseus was trea ted during the grow th phase w ith the nucleoside analogue 5-azacytidine, a powerful inducer o f cell d ifferen tia tion , as evidenced w ith animal and hum an cell lines. After induction the cell cu lture was fu rth er incubated in a production m edium in the absence o f the inducer for 10 days. T he extraction o f the freeze dried cel...
The tetraploid plants of Catharanthus roseus (L.) G. Don was obtained by colchicine induction from seeds explants, and the ploidy of the plants was identified by flow cytometry. The optimal treatment is 0.2% colchicine solution treated for 24 hours, and the induction rate reaches up to 30%. Comparing with morphological characteristics and growth habits between tetraploids and the control, we fo...
No study has been performed on identifying microRNAs (miRNAs) and their targets in the medicinal plant, Catharanthus roseus. In the present study, using the comparative genomics approach, we have predicted two potential C. roseus miRNAs. Furthermore, twelve potential mRNA targets were identified in C. roseus genome based on the characteristics that miRNAs exhibit perfect or nearly perfect compl...
While much work have been done on Catharan thus roseus (Apocynaceae) resulting in the isolation of about 100 alkaloids, there is persistent interest in the plant and new alkaloids continue to be reported. In continuation of our isolation and synthetic studies on the alkaloids of Catharanthus roseus [1 — 15], we have isolated a new alkaloid from the leaves of this plant by a combination of sele...
Gloriosa superba and Catharanthus roseus are useful in traditional medicine for treatment of various skin diseases and cancer. However, their molecular effect on psoriasis has not been investigated. In this study, the effect of ethanol extracts derived from G. superba leaves and C. roseus stems on the expression of psoriatic marker, keratin 17 (K17), was investigated in human keratinocytes usin...
Several cell lines resistant to 5-methyltryptophan were selected from wild type cells of different Catharanthus roseus suspension cultures. The resistant cells had up to 30 times the normal levels of free tryptophan. Despite the increased pool size of tryptophan anthranilate synthetase activity of resistant cells was as sensitive to inhibition by L-tryptophan as wild type cells. The overproduct...
Catharanthus roseus or vinca produces over 130 TIAs with pharmaceutical value. Two of them, namely vinblastine (VB) and vincristine (VC), are species-specific and extensively used in anticancer chemotherapeutics. To detect C. roseus genotypes abundant in desired TIAs, contrasting genotypes should be analyzed via marker-assisted selection (MAS) to avoid analytical quantitation. A trustable morph...
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