For an understanding of the molecular basis of the marked decrease in catalase activity of various tumor cells, expression of the catalase gene was studied in rat and human hepatoma cell lines and in rat liver, which was used as a control with high activity. RNA blot hybridization profiles and run-on assays indicated that the decrease in catalase activity was due to depression of catalase gene ...

Effects of melatonin on the levels of antioxidant enzymes including catalase (CAT), peroxidase (POD), glutathione reductase (GRd), and malondialdehyde (MDA), a lipid peroxidation marker, in erythrocytes of rainbow trout (Oncorhynchus mykiss) were investigated. The results showed that melatonin statistically activated the CAT, POD, and GRd activity (P < 0.05). Moreover, MDA levels were significa...

The aim of this study was to evaluate the antioxidant activity exopolysaccharides (EPS) from Lactobacillus kimchi SR8. EPS exhibited strong scavenging activities for 1,1-diphenyl-2-picrylhydrazyl and hydroxyl radicals. Two fractions (EPS-1 EPS-3) higher than EPS-2. Low-dose CEPS could significantly increase catalase (CAT) glutathione peroxidase (GSH-Px) in serum CAT, GSH-Px superoxide dismutase...

Compartmentalized chemical reactions at the microscale are important in biotechnology, yet monitoring molecular content these small scales is challenging. To address this challenge, we integrate a compact, reconfigurable reaction cell featuring electrochemical functionality with high-resolution NMR spectroscopy. We demonstrate operation of system by activity enzymes immobilized chemically disti...

Protein-based drug delivery systems have gained popularity due to their intrinsic biocompatibility, straightforward surface modification, and potential for therapeutic activity. Among proteins, enzymes are particularly attractive because of specificity, efficient reaction rates, regeneration after substrate turnover, proven track record in the treatment diseases ranging from cancer inherited me...

The objective of this study was to evaluate effect of different concentrations of catalase in two extenders on motility, viability and lipid peroxidation bull spermatozoa during semen freezing process. Thirty ejaculates collected from ten Holstein bulls were pooled and evaluated at 37 °C. Pool ejaculated was split into two main experimental groups, 1 and 2. In experiment 1, specimen was dilute...

Background and Aims:&nbsp;Cardiac hypertrophy is a compensatory augmentation response of the heart due to pressure overload that can lead to heart failure. Carvacrol is considered as the major compound of many plants, that possesses strong antioxidant properties. The present study aimed to evaluate effect of carvacrol on catalase activity and mRNA expression following left ventricular hypertrop...

Accumulation of hydrogen peroxide (H(2)O(2)) and low catalase (CAT) activity have been demonstrated in the epidermis of vitiligo patients. We investigated a possible association between the CAT exon 9 (Asp-389) gene and vitiligo susceptibility in the Turkish population. Thirty-four patients with vitiligo and 49 gender, age and ethnic matched controls were enrolled in the study. Genotyping...

In this work we investigated the inhibitory effect of Deltamethrin, Dichlorvos, Malathion and Lambdacyhalothrin on bovine liver catalase (CAT) activity. We observed that the inhibition of enzyme increased with increasing concentrations of the pesticides from 0 to 250 ppm. The Kinetics conformed to the MichaelisMenten model and a Lineweaver-Burk graph of CAT was drawn. To identify the inhibition...

INTRODUCTION Bronchial asthma is a complex disease in which genetic factors, environmental factors and oxidative damage are responsible for the initiation and modulation of disease progression. If antioxidant mechanisms fail, reactive oxygen species damage the biomolecules followed by progression of the disease. Catalase is one of the most important endogenous enzymatic antioxidants. In the pre...