introduction: in this study, the effect of para-nonylphenol as an environmental pollutant on viability, morphology and proliferation of bone marrow mesenchymal stem cells was investigated. methods: bone marrow mesenchymal stem cells of rat were treated with the 0.5, 1, 2.5, 3.5 and 5 μm of paranonylphenol for a period of 21 days, then the viability of the cells were estimated using trypan blue ...

Objective(s): Juglone is isolated from many species of the Juglandaceae family and used as an anti-viral, anti-bacterial, and anti-tumor therapeutic. Here, we evaluated juglone-induced antitumor effect in ovarian cancer SKOV3 cells. Materials and Methods: MTT assay was performed to examine juglone anti-proliferative effect. Cell cycle and apoptosis were studied using flow cytometry in juglone-t...

Introduction Thyroid cancer is a malignant disease with poor prognosis. The PI3K/Akt/mTOR and Cyclin-Dependent Kinase 4 (CDK4) pathways are vital regulators of tumor cell proliferation and survival. Therefore the present study was designed to use dual inhibition of such pathways to kill thyroid cancer cells. Methods and materials The effects of each inhibitors on human ATC and...

1. Abstract 2. Introduction 3. Materials and methods 3.1. Main reagents and cell culture 3.2. MTT assay 3.3. Comet assay 3.4. Cell cycle analysis by flow cytometry 3.5. The Caspase-3 colorimetric activity assay 3.6. Statistical analysis 4. Results 4.1. MTT assay 4.2. Comet assay 4.3. FCM assay the distribution of cell cycle and cell apoptosis 4.4. The expression of Caspase-3 5. Discussion 6. Ac...

A hallmark of Entamoeba histolytica (Eh) invasion in the gut is acute intestinal inflammation dominated by secretion pro-inflammatory cytokines. Eh contact with macrophages activates caspase-1 recruitment NLRP3 inflammasome complex a Gal-lectin and cysteine proteases 5 (EhCP-A5)-dependent manner, resulting maturation interleukin (IL)-1β IL-18. Although it well-defined that activation important ...

Introduction Thyroid cancer is a malignant disease with poor prognosis. The PI3K/Akt/mTOR and Cyclin-Dependent Kinase 4 (CDK4) pathways are vital regulators of tumor cell proliferation and survival. Therefore the present study was designed to use dual inhibition of such pathways to kill thyroid cancer cells. Methods and materials The effects of each inhibitors on human ATC and...

Purpose: To investigate the effect of eriocitrin on sevoflurane-induced neurotoxicity in mice.Methods: Mouse hippocampal neurons (HT22) were exposed to different concentrations sevoflurane for 6 h and then incubated with another 24 h. Cell viability was determined by CCK8 assay, while fluorescence intensity dichlorodihydrofluorescein used evaluate reactive oxygen species. Enzyme linked immunoso...

objective(s): protein kinase c (pkcα) is involved in modulating articular chondrocytes apoptosis induced by nitric oxide (no). hyaluronic acid (ha) inhibits nitric oxide-induced apoptosis of articular chondrocytes by protecting pkcα, but the mechanism remains unclear. the present study was performed to investigate the effects and mechanisms of pkcα regulate protective effect of hyaluronic acid....

the purpose of the present study is to investigate the cytotoxicity/apoptotic effect of 2-chloro-2′-deoxyadenosine, cladribine, (2-cda) in the human breast cancer cell line, mda-mb468 (estrogen receptor negative, er−). mtt [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2h tetrazolium bromide] assay, annexin v-fluorescein/pi and hoechst 33258 staining were used to detect cytotoxicity and cell apopto...

To investigate the effect and related mechanism of propofol anesthesia on pyroptosis in neonatal rat hippocampus. Neonatal rats were divided into a control group (injected intraperitoneally with saline), 80 mg/kg propofol) propofol+MCC950 10 MCC950) administered for 5 d. The day after end drug administration, hippocampi analyzed by transferase dUTP nick labeling assay interleukin beta-1, interl...