نتایج جستجو برای: capillary isoelectric focusing
تعداد نتایج: 141926 فیلتر نتایج به سال:
Due to the importance of monoclonal antibodies (mAb) as therapeutic agents, there is a growing demand for high resolution analytical methods to characterize these complex molecules. One important property of an mAb is its charge state that may change during and after manufacturing. A very useful technique to measure mAb charge heterogeneity is capillary isoelectric focusing (cIEF). This Applica...
For accurate micro-scale quantification of a specific protein in biological fluids, immunoaffinity chromatography (IAC) and isoelectric focusing (IEF) were combined in a single fused-silica capillary. The inner wall of the capillary was coated with an anti-E-tag antibody at the inlet side to form an IAC column, and polydimethylacrylamide, a neutral polymer, at the outlet side to form the capill...
Sensitivity enhancement of enzyme-linked immunosorbent assay (ELISA) in capillary-based microdevice was achieved by the concentration of fluorescent final enzyme reaction product using capillary-isoelectric focusing (CIEF). In order to realize this, fluorescent substrate molecule, which can change its structure into ampholyte ion after an enzyme reaction, was newly synthesized and used. Approxi...
Capillary electrophoresis (CE) has emerged as a powerful technique for comprehensive physicochemical characterization of monoclonal antibodies (mAbs) well other therapeutic modalities. The method provides high resolution separation and sensitivity analysis biomolecules. CE based techniques such sodium dodecyl sulphate capillary gel (SDS-CGE, also referred to SDS), zone (CZE) imaged isoelectric ...
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