methods the current study generated two recombinant lentiviruses, plex-gcn and plex-gcp, bearing noxa and puma, respectively. transduction of both genes to hepatocarcinoma (hepg2) was verified using fluorescent microscopic analysis, western blotting, and quantitative real-time polymerase chain reaction (pcr). to evaluate the potential of noxa and puma to initiate apoptosis, a caspase-9 real-tim...

background: recently, the use of t7 rna polymerase instead of other viral and cellular promoters is increasing due to high efficacy of transcription in the cell cytoplasm by this polymerase. in order to translate the transcripts produced by t7 rna polymerase in mammalian cell lines, it is necessary to include internal ribosome entry site (ires) sequences. in addition, if sequence of poly a sign...

Despite the major advances in conventional forms of treatment (i.e. surgical techniques, radiotherapy and chemotherapy) and improved survival rates, cancer is still the second leading cause of death in developing countries. One major limitation of cytotoxic drugs and radiation in the treatment of cancer patients is their inability to discriminate between malignant and normal tissues. This in tu...

abstract accumulation of gene changes and chromosomal instability in response to cellular dna damage lead to cancer. dna damage induces cell cycle checkpoints pathways. checkpoints regulate dna replication and cell cycle progression, chromatin restructuring, and apoptosis. checkpoint kinase 2 (chk2) is activated in response to dna lesions. atm phosphorylate chk2. the activated chk2 kinase can p...

background: radioiodine therapy has proven to be an effective method in the treatment of patients with differentiated thyroid carcinoma after thyroidectomy. the scope of this study is to describe a method to obtain the dose of organs using medical internal radiation dosimetry (mird) method. at the end, the results of mird calculations were compared with thermoluminescent dosimeter (tld-100). ma...

introduction: acoustic transient cavitation is the primary mechanism of sonochemical reaction and has potential use for tumor treatment. in this study, the in vivo anti-tumor effect of simultaneous dual-frequency ultrasound at low-level intensity (isata < 6 w/cm2) was investigated in a spontaneous murine model of breast adenocarcinoma in balb/c mice. materials and methods: forty tumor bearing m...

objectives:this study used a quasi-experimental method inthe pretest-posttest to evaluate the efficacy of cognitive-existential group therapy on hopefulness in parents of children with cancer. methods:thirty parents (24 to 54 years old) of children with cancer were divided randomly into experimental and control groups. both groups were assessed with miller hope scale (1988), as pre-test. the ex...

personalized medicine is a set of diagnostic, prognostic and therapeutic approaches in which medical interventions are carried out based on individual patient characteristics. as life expectancy increases in developed and developing countries, the incidence of diseases such as cancer goes up among people in the community. cancer is a disease that the response to treatment varies from one person...

context: vomiting is one of the most prevalent side effects of chemotherapy in cancer patients. aims: the aim of this study was to evaluate the effect of ginger plant on chemotherapy-induced vomiting, since the previous studies were somehow imperfect and have provided controversial results. materials and methods: this randomized double-blind placebo-controlled clinical trial was conducted on 80...