Peony is a traditional Chinese flower with significant ornamental and medicinal value. However, there are still problems, such as serious browning, difficulties in differentiation, rooting low regeneration efficiency the process of system established, which have hindered development transgenic peony technology. Establishing an efficient considered to be important goal among researchers. Here, w...

The first experiment was done to select the most suitable explant establish a culture for shoot regeneration. Four different explants (cotyledon, cotyledon node, leaf and hypocotyl) excised from in vitro grown seedlings were cultured on MS medium containing 1.0 mg l-1 BAP 0.2 NAA. Cotyledon responsive which exhibited quick callus initiation. second conducted determine effect of media BAP, NAA a...