The mineral nutrient requirements of Pinus taeda cells were explored using quantitative cell culture growth measurements. An appraisal was thereby made of the critical features of a novel and successful medium which was developed specifically for this gymnosperm using chemical composition data for developing seeds, and characterized by generally high concentration of all micronutrients, high ma...

Adequate iodide supply and metabolism are essential for thyroid hormones synthesis. In thyrocytes, iodide uptake is mediated by the sodium-iodide symporter, but several proteins appear to be involved in iodide efflux. Previous studies demonstrated that pendrin is able to mediate apical efflux of iodide in thyrocytes. Acute iodide excess transiently impairs thyroid hormone synthesis, a phenomeno...

The present study is aimed to determine the role of calcium signaling evoked by the oxygen radical, hydrogen peroxide (H2O2) and the specific inhibitor of calcium reuptake thapsigargin on apoptosis in the human leukemia cell line HL-60. Our results show that treatment of HL-60 cells with 100 µM H2O2 and 1 µM thapsigargin induced a transient increase in cytosolic free calcium concentration ([Ca(...

OBJECTIVE Oxidized low-density lipoprotein (oxLDL)-induced apoptosis of vascular endothelial cells may contribute to plaque erosion and rupture. We aimed to clarify the relationship between the oxLDL-induced calcium signal and induction of apoptotic pathways. METHODS AND RESULTS Apoptosis was evaluated by biochemical methods, including studies of enzyme activities, protein processing, release...

a simple, mild and practical procedure has been developed for the synthesis of symmetrical and unsymmetrical ethers by using dmso, tbai in the presence of k2co3. we extended the utility of  potassium carbonate as an efficient base for the preparation of ethers. a wide range of alkyl aryl and dialkyl ethers are synthesized from treatment of aliphatic alcohols and phenols with various alkyl halid...

a sensitive and selective extraction - spectrophotometric method for the determination of trace amounts of palladium is reported. the method is based on quantitative extraction of brownish tetrabutylammoium tetraiodopalladium (ii) ion pair into a small valume of chloroform at ph 1.3-12.0, followed by spectrophotometric measurements. the beer's law is obeyed over the range 0.02-1.09 mgml-1 at 44...

Iodide (I(-))-accumulating bacteria were isolated from marine sediment by an autoradiographic method with radioactive (125)I(-). When they were grown in a liquid medium containing 0.1 microM iodide, 79 to 89% of the iodide was removed from the medium, and a corresponding amount of iodide was detected in the cells. Phylogenetic analysis based on 16S rRNA gene sequences indicated that iodide-accu...