BACKGROUND Calcitonin has been demonstrated to have chondroprotective effects under pre-clinical settings. It is debated whether this effect is mediated through subchondral-bone, directly on cartilage or both in combination. We investigated possible direct effects of salmon calcitonin on proteoglycans and collagen-type-II synthesis in osteoarthritic (OA) cartilage. METHODS Human OA cartilage ...

We characterized rabbit antisera to synthetic human calcitonin that specifically recognize the aminoand carboxy-terminal regions of calcitonin (antisera 1-506 and HMS-385, respectively), by both classical (parallelism of dose-response curves) and (owing to the unavailability of a variety of analog fragments of human calcitonin) biochemical plus enzymic alteration of synthetic human calcitonin. ...

1. Role of c-AMP: Calcitonin binds to specific calcitonin receptors on the plasma membrane of bone osteoclasts and renal tubular epithelial cells, activates adenyl cyclase which increase c-AMP level which mediates the cellular effect of the hormone. This is the principal mechanism by which calcitonin acts. 2. Celluar Shift: It has been suggested that calcitonin may directly affect the relative ...

A sensitive, specific radioimmunoassay of calcitonin is described, suitable for measuring the hormone in peripheral plasma of normal man. With this assay we have demonstrated that calcitonin is detectable under basal Conditions and the concentrations respond to induced, and spontaneous hypercalcemia and induced hypocalcemia. The mean value for basal calcitonin of normal men was 182 pg/mI (range...

OBJECTIVES Implantation failure is an obstacle in assisted reproduction techniques (ART). Calcitonin is a molecules involved in uterine receptivity and embryo implantation. Melatonin can promote embryo quality and improve implantation. This study examines the effect of pretreatment of blastocysts with melatonin and calcitonin on heparin binding-epidermal growth factor (HB-EGF) expression in mur...

A strategy is presented for identification of putative neurohormones that were predicted by sequence analysis of recombinant cDNa molecules. The nucleotide sequence of a cloned calcitonin cDNA insert predicts that the proteolytic excision of calcitonin from a 136-amino acid precursor should generate two additional peptides derived from regions flanking the calcitonin sequence on the NH2 and car...

Calcitonin mRNA and calcitonin gene-related peptide (CGRP) mRNA both are generated from the calcitonin gene because of tissue-specific alternative splicing of the primary transcript. It is currently established that, of the two mature transcripts, calcitonin mRNA is far the predominant transcript produced in thyroid C-cells whereas only CGRP mRNA is produced in the nervous system. However, here...

Effects of [Asu1,7]-eel calcitonin on gastric somatostatin and gastrin secretion were studied by using the isolated perfused rat stomach. [Asu1,7]-eel calcitonin (10(-9)--10(-7)M) caused a simultaneous dose-dependent increase of gastric somatostatin release and decrease of gastrin secretion, with a significant correlation between these two. The demonstration of calcitonin stimulation of gastric...

Objective(s): As heat, pain is one of the most common clinical symptoms. Generally, calcitonin (CT) is prescribed as an analgesic agent for the treatment of pain, especially for the pain caused by osteoporosis or primary and metastatic bone tumor. However, the detailed mechanism remains unknown.Materials and Methods: In this study, chronic constriction injury (CCI) rat model was created, and ho...

Twenty-three out of 28 patients with metastatic breast carcinoma and one out of 13 patients with localised disease had raised levels of plasma immunoreactive calcitonin. Monolayer cultures of breast carcinomas maintained for up to 10 weeks released immunoreactive calcitonin, and a primary breast carcinoma passaged in "nude" mice for over a year contained material immunologically and chromatogra...