Carcinomas of the exocrine pancreas are poorly understood and have a poor prognosis because of their highly malignant nature. Using two human pancreatic cancer cell lines, AsPc-1 and Capan-2, we have investigated avenues that might be useful in targeting the delivery of antineoplastic agents to such cancers. Qualitative RNA PCRs established the presence of the oligopeptide transporter PEPT 1 in...

Gut-derived adenocarcinoma Caco-2 cells were treated with sodium butyrate (NaB) at physiologically relevant concentrations. We characterized its effects on proliferation, differentiation, apoptosis, adhesion to the solid support and interleukin-8 secretion. Differentiation was determined by brush border alkaline phosphatase activity. Apoptosis was assessed by acridine orange and Hoechst stains....

Background and objectives: In review of traditional Persian medicine (TPM) literature concerning multi drug therapy, a group of medicinal plants that are called "convoy drugs", agents which penetrate fast into whole or specific part of the body and accelerate delivery of drugs into specific target has been mentioned. In this study, the inhibitory effect of the aqueous extracts ...

The aim of this study was to assess the modulatory effect TcpA in expression CEACAM1 adhesin molecule and IL-1, IL-8, TNF-α pro-inflammatory cytokines Coculture model Caco-2/PBMC (peripheral blood mononuclear cell) that can mimic intestinal milieu. gene from Vibrio cholerae ATCC14035 cloned pET-28a transformed into Escherichia coli Bl-21. recombinant TcpA-His6 protein expressed purified using N...

To determine a suitable condition for in vitro infection model of Cryptosporidium parvum, four different cell lines, AGS, MDCK, HCT-8 and Caco-2, were used as host cell lines which were cultured at various concentrations of added supplements. These supplement include fetal bovine serum (FBS), sodium choleate, ascorbic acid, folic acid, calcium pantothenate, para-aminobenzoic acid and pyruvate a...