The effects of holding diluted ram semen at 5 ◦C for up to 48 h prior to cryopreservation were investigated. Semen from six rams was collected by electro-ejaculation in the autumn and again from six different rams in the spring. The sperm concentration and motility were determined using spectrophotometry and computerized automated semen analysis, respectively. Samples were diluted at 23 ◦C to 4...

Human monocytic cell line THP-1 incubated with as little as 10 ng/ml of phorbol myristate acetate bound and metabolized 1-2 micrograms of Ac-LDL over a 5-h period. In the absence of phorbol treatment, no specific metabolism of Ac-LDL occurred. Optimal levels of receptor were reached after 72 h of exposure. Induction of receptor was dependent on protein and RNA synthesis and was partially revers...

Avemar (MSC) is a nontoxic fermented wheat germ extract, which has been shown to significantly improve the survival rate in patients suffering from various malignancies. We investigated its effects in sensitive and 5-FdUrd/Ara-C cross-resistant H9 human lymphoma cells. After 48 and 72 h of incubation, Avemar inhibited the growth of sensitive H9 cells with IC50 values of 290 and 200 microg/ml, w...

BACKGROUND Consumption of fruit and vegetables is associated with improved health and a decreased prevalence of chronic degenerative processes. OBJECTIVES The objectives were to assess the bioavailability of vitamin C from orange juice and its influence on plasma vitamin C and 8-epi-prostaglandin F(2 alpha) (8-epi-PGF(2 alpha)) concentrations in a healthy human population. DESIGN Six men an...

Trichomoniasis is one of the most common urinary-genital tract disease. The causal agent of trichomoniasis is flagellate protozoan called Trichomonas vaginalis. Metronidazole and tinidazole are the drug of choice for treatment. Carcinogenic and embryo-teratogenic effects of the mentioned drugs and resistance to metronidazole has been reported. According to cytotoxic effects of medicinal herbs i...

OBJECTIVE To study the mechanism of early chemosensitivity and later chemoresistance of Cytarabine (Cyt) to HL-60 cells to regulate ERK/Slug signal pathway. MATERIALS AND METHODS HL-60 cells were stably transfected with NF-κBp65 siRNA，P53 up-regulated modulator of apoptosis (PUMA) siRNA (PUMA siRNA) and Slug siRNA respectively or exposed to 25 nM U0126 for 24h, then the cells were exposed to ...

1 : A solution of 9 [25] (22 mmol) in Et,O (40 mL) was added slowly over 15 min at 0 -C to a solution of phenylsilane (2.4 g, 22 mmol) in Et,O (40 mL). The mixture was stirred at room temperature for 2 h and then hydrolyzed at 0°C with 0.4 mL ofH,O. After filtration on celite. distillation gave 5.4 g (83%) of 1 as a colorless oil. B.p. 115-117 'C/0.02Torr; 'HNMR (250 MHz, CDCI,, TMS):6 = 2.0(s....

TAB-250 and BACH-250 are murine and human chimeric antibodies directed at the extracellular domain of the gp185c-erb-2 (HER2/neu) growth factor receptor overexpressed in a variety of tumor types, including ovarian and breast carcinoma. The ribosome-inhibiting plant toxin gelonin (rGel) was chemically coupled to both antibodies, and the resulting immunotoxins were purified and tested in vitro ag...

Nikkomycin Z is an antifungal drug that inhibits chitin synthase. This agent is under development as an orphan product for treatment of coccidioidomycosis. Safety and pharmacokinetics of nikkomycin Z were evaluated in healthy male subjects following single, rising oral doses ranging from 250 mg to 2,000 mg. A total of 12 subjects were recruited and divided into two groups. Group 1 (n = 6) recei...

BACKGROUND Extracorporeal carbon dioxide removal has been proposed to achieve protective ventilation in patients at risk for ventilator-induced lung injury. In an acute study, the authors previously described an extracorporeal carbon dioxide removal technique enhanced by regional extracorporeal blood acidification. The current study evaluates efficacy and feasibility of such technology applied ...