To prove that the peptidic HIV-1 fusion inhibitors containing the pocket-binding domain (PBD) mainly target the hydrophobic pocket in the gp41 N-terminal heptad repeat (NHR), we constructed pseudoviruses by replacement of Q64 in the gp41 pocket region with Ala (Q64A) or Leu (Q64L). These viruses were highly resistant to C34 and CP32M containing the PBD, while they were susceptible to T20 (enfuv...

Here, we described the production of a cellulase-free alkaline xylanase from Bacillus pumilus MTCC 5015 by submerged fermentation and its application in biobleaching. Various process parameters affecting xylanase production by B. pumilus were optimized by adopting a Plackett-Burman design (PBD) as well as Response surface methodology (RSM). These statistical methods aid in improving the enzyme ...

OBJECTIVE Drawing on the Theory of Planned Behavior (TPB) and the Transtheoretical Model (TTM), this study (1) examines links between stages of change for following a plant-based diet (PBD) and consuming more fruits and vegetables (FV); (2) tests an integrated theoretical model predicting intention to follow a PBD; and (3) identifies associated salient beliefs. DESIGN Cross-sectional. SETTI...

Abstract Biosurfactants are bio-based amphiphilic molecules with extensive applications in various industries. These eco-friendly alternatives possess numerous advantages over chemical surfactants. However, high production costs hinder market competitiveness of biosurfactants. Production synthetic surfactants range between $1-3/kg, while biosurfactants cost $20-25/kg. Principal challenges hinde...

The rapid growth of the urban population and associated environmental concerns are challenging city planners developers to consider sustainable cost-efficient building systems. Timber-based buildings, such as systems, increasingly used. timber however, being lighter flexible, can be vulnerable earthquakes wind loads. This paper gives a state-of-the-art review on performance-based design (PBD) c...

        Sensitivity analysis of structures considering their nonlinear behavior has recently been an important process in optimal design of them based on new design concepts such as Performance-Based Design (PBD). The main objective of this research is to develop an efficient and applicable method for nonlinear sensitivity analysis of Reinforcement Concrete Moment Resisting Frames (RCMRF) accou...

This paper describes optimization method that combines the Plackett-Burman design, a factorial design and the response surface method, which were used to optimize the medium for the production of L-asparaginase by Serratia marcescens SB08. Four medium factors, from out of 11 medium factors, were screened by Plackett-Burman design experiments and subsequent optimization process to find out the o...

Mevastatin, also known as compactin or ML-236B is a member of the class of statins belonging to the polyketide group. Polyketides are rich sources of pharmaceuticals, including antibiotics, anticancer drugs, and cholesterol-lowering drugs, immunosuppressants and other therapeutics [1]. This study was performed for the production of mevastatin using sesame oil cake as substrate with Pencillium c...

OSINT stands for Open Source Intelligence, (O)SI for (Open) Social Intelligence, PbD for Privacy by Design. The CAPER project has built an OSINT solution oriented to the prevention of organized crime. How to balance freedom and security? This position paper describes a way to embed the legal and ethical issues raised by the General Data Reform Package (GDRP) in Europe into this kind of surveill...

BACKGROUND Polo-like kinase-1 (Plk1) plays a crucial role in cell proliferation and the inhibition of Plk1 has been considered as a potential target for specific inhibitory drugs in anti-cancer therapy. Several research groups have identified peptide-based inhibitors that target the polo-box domain (PBD) of Plk1 and bind to the protein with high affinity in in vitro assays. However, inadequate ...