Introduction: Oxidative stress (OS) is an important factor in the etiology of male infertility which presents abnormal increase in the production of free radicals with negative influences on reproduction. The aim of present study is evaluation effect of mercury (Hg) (600& 1200 micro molar) and nicotine (0.75 mM), in vitro, on membrane integrity (LPO), GSH content, motility, and acrosome reactio...

Fertility potential of spermatozoa depends on maintenance of the mitochondrial membrane potential ( m) that provides energy for sperm hyperactivation immediately prior to successful fertilization. Mitochondrial structure and integrity are associated with sperm motility and reduced fertility, and measurement of m may be suitable for determining bull semen quality. Mitochondrial membrane potentia...

The effects of potassium, sodium, chloride and calcium on the percentage progressive motility of samples of chimpanzee and bull spermatozoa were investigated. Inhibitors and stimulatory of motility were identified. Three parameters of chimpanzee sperm motility, velocity, frequency and amplitude, were measured. There was no consistent change related to ion concentration. The ionic constituents o...

Clusterin is a major protein in bull reproductive tract secretions and sperm membrane extract. A polyclonal antibody was produced against clusterin from bull cauda epididymal fluid (CEF) and used for the localization of the protein on bull spermatozoa. Immunoblotting of unreduced bovine samples showed that the anticlusterin antibody reacted with a protein of approximately 94- to 100-kd in rete ...

The surface expression of CD9 (cluster-of-differentiation antigen-9) in sperms of certain mammalian species has been attributed to its fusion with the egg and thereby dictating the fertility of species. In the present study, we investigated the association of CD9 with crossbred bull sperm quality and quantity trait was analyzed using a total of 96 Frieswal (HF × Sahiwal) crossbred. A single nuc...

The chemical nature of the factors that induce the selective fusion and vesiculation of the outer acrosomal and plasma membranes in sperm cells is yet to be established [ 1,2]. This process, known as the acrosome reaction, precedes fertilisation of the egg and is accompanied by the release of the degradative enzymes sequestered in the acrosomal vesicle of sperm [l]. The synthetic tripeptide, fo...

This paper aimed at investigating the potential use of sperm DNA fragmentation (SDF) to improve the routine screening of infertility of Holstein bulls. Cryopreserved sperm samples from 201 Holstein bulls provided by an AI center were used in the analyses of SDF at 0 (SDF_0) and 6 (SDF_6) h of incubation at 37oC. A refi nement of the sperm chromatin dispersion test implemented in the Sperm-Halom...

Bull effect on results of in vitro embryo production has been well documented. The aim of the present study was to find the relationship between quality of bull sperm chromatin and its effect on in vitro embryo production. Bovine in vitro matured oocytes were fertilized in vitro using capacitated spermatozoa (freshly ejaculated or frozen-thawed) of 12 bulls. Semen was simultaneously processed a...

1. Methods of homogenizing suspensions of washed mammalian spermatozoa were studied. The most useful methods were those using sonication and those using a French press. 2. Hexokinase, phosphofructokinase, glucose phosphate isomerase and adenosine triphosphatase activities in ram, bull and boar spermatozoa were investigated by using these two homogenization methods. Glucose phosphate isomerase, ...