perishability of blood products as well as uncertainty in demand amounts complicate the management of blood supply for blood centers. this paper addresses a mixed-integer linear programming model for blood platelets production planning while integrating the processes of blood collection as well as production/testing, inventory control and distribution. whole blood-derived production methods for...

OBJECTIVE We investigated the capacity of microRNAs isolated from peripheral blood buffy coat collected late during the first trimester to predict preeclampsia. STUDY DESIGN The cohort study comprised 48 pregnant women with the following pregnancy outcomes: 8 preeclampsia and 40 with normal delivery outcomes. Quantitative rtPCR was performed on a panel of 30 microRNAs from buffy coat samples ...

The prevalence rate of Trypanosoma brucei infection in pigs was appraised by a monoclonal antibody-based antigen-detection enzyme immunoassay (antigen-ELISA). Blood samples were collected in the abattoir from pigs reared in the rain forest and derived savannah region of Nigeria under the traditional extensive management system. Blood samples were also collected from 50 exotic pigs reared on a c...

Bovine buffy coat cells infected with the bovine leukemia virus (BLV) induce syncytia formation in human diploid embryonic lung cells as well as in monolayer cell cultures of bovine, simian, ovine, bat, and caprine origin, but not in mouse fibroblast cells, normall rat kidney cells, or RSV-transformed rat cells. Syncytia were not observed in diploid embryonic lung cells inoculated with bovine b...

Interest in genome-wide association studies to identify susceptibility alleles for cancer is growing, and several are currently planned or under way. Although the feasibility of collecting buccal cell samples as an alternative to venous blood samples as a source of genomic DNA has been shown, the validity of using DNA from buccal cells for genome-wide scans has not been assessed. We used 46 pai...

BACKGROUND In the present study, different methods for preparation of platelet-rich plasma (PRP) are investigated in order to standardize the component in terms of growth factor content. The effects of concentration technique and storage duration are also analyzed. METHODS PRP was collected from 40 donors by plateletpheresis as well as by the buffy coat and tube method. Concentration of growt...

BACKGROUND Culture of S. Typhi is necessary for the definitive diagnosis of typhoid fever and provides isolates for antibiotic susceptibility testing and epidemiological studies. However, current methods are not fully optimised and sourcing culture media and bottles for culture media may be problematic. METHODOLOGY In two hospital laboratories in Viet Nam, comparisons of media for blood and s...