This study examined flock size and management, level of internal and external parasite burden and seroprevalence of antibodies to poultry pathogens in indigenous chickens in Bokaa village, Kgatleng district, Botswana. The mean flock size was 22.6 +/- 6.85 with a range of 11-34. The mean body weights of cocks and hens were 2.28 +/- 0.56 kg and 1.70 +/- 0.38 kg, respectively. Housing and commerci...

background and objectives: brucellosis is an important health problem in developing countries and no vaccine is available for the prevention of infection in humans. because of clinically infectious diseases and their economic consequences in human and animals, designing a proper vaccine against brucella is desirable. in this study, we evaluated the immune responses induced by a designed recombi...

The antiglobulin consumption test of Steffen (1954) has been adapted for the detection of Brucella antibodies in human serum. This screening method takes approximately two hours to complete and sera which give a negative result need no further investigations, whilst positive sera should be fully tested by the conventional Brucella serology technique.

Aqueous glutaraldehyde has been polymerized under alkaline conditions in the presence of a surfactant to yield microspheres of varied diameters. Microbeads of a nominal 300 nm diameter, made fluorescent via fluoresceinisothiocyanate, were used as a carrier of rabbit anti-human red blood cell antibodies. Specific labeling of the human red blood cells as well as diagnostic use of these fluor...

Ten striped dolphins, Stenella coeruleoalba, stranded along the Costa Rican Pacific coast, had meningoencephalitis and antibodies against Brucella spp. Brucella ceti was isolated from cerebrospinal fluid of 6 dolphins and 1 fetus. S. coeruleoalba constitutes a highly susceptible host and a potential reservoir for B. ceti transmission.

An enzyme immunoassay (EIA) was developed for detecting Brucella antibodies in milk of cows infected with Brucella abortus. The enzyme immunoassay using heat-killed cells of B. abortus strain 19 was of comparable sensitivity to the Brucella ring test in detecting antibodies in milk of a reference positive control cow experimentally infected with B. abortus strain 2308 and in milk of 16 naturall...

Brucellosis is a zoonotic disease which can be transmitted by direct or indirect contact with infected animal or their products. It is an important public health problem but little is known on brucellosis in the Malaysian population. The aim of this study was to determine the presence of Brucella antibodies using commercial Brucella IgG and IgM ELISA kits (Vircell, SL, Barcelona Spain). A total...

background and objective: brucellosis is a zoonotic disease of worldwide distribution and has great economic importance. despite its control in many countries, it remains endemic in iran. brucellosis was investigated in many high risk occupational groups; however, few studies on the prevalence of brucellosis among blood donors are available. to determine the seroprevalence of brucellosis antibo...

A total of 183 cattle comprising 54 bulls, 53 milking cows, 76 heifers and 44 calves in the Akwapim-South district of Ghana were tested for antibodies against Brucella abortus using the Rose Bengal plate test. The results indicated that cattle in the Akwapim-South district were infected with Brucella with a mean seroprevalence of 6. 6%. There was no difference in the seroprevalence either betwe...

background and objectives: brucella melitensis infection is still a major health problem for human and cattle in developing countries and the middle east. materials and methods: in this study, in order to screen immunogenic candidate antigens for the development of a brucella subunit vaccine, a cytoplasmic protein (dnak) and an outer membrane protein (omp31) of b. melitensis were cloned, expres...