Receptor Associated Protein 80 (RAP80) is a subunit of the BRCA1-A complex and targets BRCA1 to DNA damage sites in response to DNA double strand breaks. Since mutations of BRCA1 are associated with familial ovarian cancers, we screened 26 ovarian cancer-derived cell lines for RAP80 mutations and found that TOV-21G cells harbor a RAP80 mutation (c.1107G >A). This mutation generates a stop codon...

BACKGROUND The tumor suppressor BRCA1 plays a pivotal role in maintaining genomic stability and tumor suppression. The BRCA1-A complex is required for recruitment of BRCA1 to DNA damage sites, DNA repair and cell cycle checkpoint control. Since germline mutations of BRCA1 often lead to breast tumors that are triple-negative breast cancer (TNBC) type, we aimed to investigate whether genetic defi...

Yan et al. describe how a deubiquitinating enzyme complex regulates the mitotic spindle assembly factor NuMA. BRCC36 is a de-ubiquitinating enzyme that preferentially cleaves lysine 63–linked poly-ubiquitin chains. As part of the Rap80 complex, BRCC36 regulates the repair of DNA double-strand breaks, but the enzyme can also assemble into a distinct complex, known as BRISC. Yan et al. found that...