artemisia annua is still the only commercial source of artemisinin. to date, a number of biochemical and molecular studies about artemisinin’s biosynthetic pathway have been carried out. in metabolic engineering approach, isolation and characterization of promoters leads to an understanding of which cis-acting elements are responsible for the regulation of gene expression. dbr2 is a key enzyme ...

We solve principal component regression (PCR), up to a multiplicative accuracy 1+γ, by reducing the problem to Õ(γ−1) black-box calls of ridge regression. Therefore, our algorithm does not require any explicit construction of the top principal components, and is suitable for large-scale PCR instances. In contrast, previous result requires Õ(γ−2) such black-box calls. We obtain this result by de...

Uso de fungicidas em cultivos feijao apresenta grande potencial contaminacao a saude humana e do solo. Em virtude disso, faz-se necessario introducao desenvolvimento novos agentes biologicos ambientalmente seguros. Sendo assim, podem-se destacar as bacterias genero Bacillus visto que possuem um alto valor biotecnologico da variedade compostos multiplicidade mecanismos antifungicos. Desta forma,...

many recent studies have shown that glycosylation patterns of agaricus bisporus are similar to those of mammalians, so that this organism is a good candidate for the expression of glycosylated pharmaceutical protein. to achieve constant interested gene expression in all cells of the organism, proper promoter isolation is necessary. to isolate this promoter, pcr with specific primers was perform...

Rice is the primary staple food for more than half of the world’s population but very sensitive to various abiotic stresses (submergence) causing crop loss. During abiotic stresses various genes are differentially expressed to cope up with the stress conditions. The identification of Sub1A gene was a major breakthrough for the submergence tolerance which often regulates other genes by binding t...

Two-step PCR procedures are an efficient and well established way to generate amplicon libraries for NGS sequencing. However, there is a high risk of cross-contamination by carry-over of amplicons from first to second amplification rounds, potentially leading to severe misinterpretation of results. Here we describe a new method able to prevent and/or to identify carry-over contaminations by int...

A total of 62 Pseudomonas aeruginosa strains isolated from two hospitals in Siedlce (Poland) were studied by repetitive element based PCR (rep-PCR) using BOX primer. BOX-PCR results revealed the presence of 7 numerous genotypes and 31 unique patterns among isolates. Generally, the strains of P. aeruginosa were characterized by resistance to many antibiotics tested and by differences in serogrou...