Abbreviations: BSA: bovine serum albumin CHO: Chinese hamster ovary CL: total plasma clearance Cmax: maximum plasma concentration ELISA: enzyme-linked immunosorbent assay FCS: fetal calf serum ip: intraperitoneal iv: intravenous PBS: phosphate-buffered saline rhGM-CSF: recombinant human granulocyte-macrophage colony-stimulating factor t1/2 α: distribution half-life t1/2 : β terminal half-life T...

This study investigated interrelationships between the bovine ovarian cycle and white blood cells and tested the hypothesis that the ovary produces collagen-like materials with leukocyte attractant activity. We examined the in vitro secretion of leukocyte attractant activity by peri-ovulatory ovarian tissues and evaluated the leukocyte attractant potential of some ovarian biochemicals. Fluid fr...

BACKGROUND Nucleoplasmin 2 (NPM2) is an oocyte-specific nuclear protein essential for nuclear and nucleolar organization and early embryonic development. The aims of this study were to clone the bovine NPM2 gene, determine its temporal expression during oocyte development and early embryogenesis, and evaluate the potential role of miRNA-181a in regulation of its expression. METHODS A 329 bp c...

Sulfated mucopolysaccharides, such as heparin, heparan sulfate, chondroitin sulfates and dermatan sulfate, have been shown to be normal constituents of animal tissues and body fluids. Their biological functions are not yet known with certainty. A role as lubricants in the living organism was ascribed to these sulfated mucopolysaccharides due to their highly viscous and elastic properties in sol...

Studies of selenium (Se) status indicate that Se is necessary for fertility but how precisely is not known. We aimed to show that Se was important in bovine female reproductive function. The elemental distribution in the bovine ovary (n = 45 sections) was identified by X-ray fluorescence (XRF) imaging. Se was consistently localized to the granulosa cell layer of large (>10 mm) healthy follicles...

Adiponectin is one of the most important, recently discovered adipocytokines that acts at various levels to control male and female fertility through central effects on the hypothalamus-pituitary axis or through peripheral effects on the ovary, uterus, and embryo. We studied simultaneous changes in the gene expression pattern of adiponectin and adiponectin receptors 1 and 2 (AdipoR1 and AdipoR2...

A complete genomic clone and a full-length cDNA coding for bovine intestinal alkaline phosphatase have been isolated and sequenced. The gene (5.4 kb) contains 11 exons separated by ten small introns at positions identical to those other members of the eukaryotic tissue-specific alkaline phosphatase family. In addition, 1.5 kb of upstream sequences contain putative regulatory elements showing se...