the objective of this study was to compare different antigen preparations to produce monoclonalantibodies against bovine leukaemia virus gp51su. the four antigen preparations for immunization ofbalb/c mice were: cl: blv-flk cell lysate, uf: a fraction of cl (between 30 and 100 kda), wvp: wholevirus particles and sp: with ion exchange chromatography, gp51su was semipurified. a total of ninesucce...

BIV is a well-known bovine immunosup-pressive cause, but its pathogenesis has not been well characterized. It seems that it is possible that cofactors such as co- infection with other bovine viral pathogens may play a role in enhancing the pathogenesis of BIV infection; BVDV also has immunosuppressive effects. OBJECTIVE: The aim of this study was determination of possible correlation between BI...

A seroprevalence study of bovine immunodeficiency virus (BIV) was undertaken on 1,541 serum samples from Holstein cattle from 23 herds, located in different geographical regions of Poland. The analysis was performed using ELISA, with recombinant Gag protein of BIV as antigen. The average BIV prevalence was 4.9% in individual cattle, while the percentage of herds harboring at least one seroposit...

A 120-amino-acid polypeptide selected from the transmembrane protein region (tTM) and the major capsid protein p26 of bovine immunodeficiency-like virus (BIV) were expressed as fusion proteins from recombinant baculoviruses. The antigenic reactivity of both recombinant fusion proteins was confirmed by Western blot with bovine and rabbit antisera to BIV. BIV-negative bovine sera and animal sera ...

background and objective: anemia is a common manifestation of human immunodeficiency virus (hiv) infection, occurring in approximately 30% of patients with asymptomatic infection and in as many as 75% to 80% of those with aids. anemia has been associated with decreased quality of life and decreased survival. in this study we aimed to determine the prevalence and related factors of anemia in hiv...

background and objectives: hiv infected patients are at risk for vaccine-preventable infections such as tetanus and diphtheria (td). in these patients, these antibodies tend to decline faster. due to the limited data, this study assessed the seroprevalence of tetanus and diphtheria antibodies in hiv infected patients in tehran, iran. materials and methods: this case-control study was carried o...

background: hemophilia a and b are the most frequent congenital coagulation disorders. this study was conducted to determine the prevalence of hepatitis b, c and human immunodeficiency viruses among hemophilic patients in hamadan, iran. patients and methods: in this study, patients with hemophilia a and b treated in hamedan hemophilia center, hamedan, iran, were screened for hepatitis b, c and ...

Heterophile antibodies are a well-recognized cause of erroneous results in immunoassays. We describe here a 22-month-old child with heterophile antibodies reactive with bovine serum albumin and caprine proteins causing false-positive results to human immunodeficiency virus type 1 and other infectious serology testing.

Lentivirus envelope glycoproteins have unusually long cytoplasmic domains compared to those of other retroviruses. To identify cellular binding partners of the simian immunodeficiency virus (SIV) envelope transmembrane protein (gp41) cytoplasmic domain (CD), we performed a yeast two-hybrid screen of a phytohemagglutinin-activated human T-cell cDNA library with the SIV gp41 CD. The majority of p...