Reports that Clostridium botulinum toxin can sometimes be detected in the absence of indicators of overt spoilage led to a systematic study of this phenomenon in a model system. Media with various combinations of pH (5.0 to 7.0) and glucose (0.0 to 1.0%) were inoculated with vegetative cells of C. botulinum 62A and incubated anaerobically at 35 degrees C. Although growth and toxin production oc...

umber of clostridial bacteria produce toxins which ADP-ribosylate monomeric actin. These include botulinum C2 toxin (2), Clostridiumperfringens iota toxin (23, 26), Clostridium spiroforme toxin (20, 27), and an ADP-ribosyltransferase produced by Clostridium difficile (21). The toxins have turned out to be valuable tools for investigating the actin cytoskeleton since ADP-ribosylation of actin bl...

OBJECTIVE To develop and evaluate tools for the audit of spasticity management with botulinum toxin. DESIGN Audit tools to assess the organisation of services and process of care were developed with a consensus process. The tools were piloted across 8 services using a retrospective case note audit. Inter-rater reliability was assessed, using percentage agreement and kappa scores. Clinicians i...

There are many medical and dental conditions which do not have complete treatment modalities in conventional ways. The botulinum toxin can be used as an alternative treatment modality working through chemo denervation method in many medical and dental conditions. An internet research was done for botulinum toxin used in dentistry and all articles and studies were selected, articles related to d...

Botulinum toxin type B was studied for the management of hyperkinetic facial lines. It showed clinical longevity of up to 12 weeks on subjective rating scales and 8 weeks on objective rating scales. Botulinum toxin type B has shown relative safety but can have autonomic side effects at higher total doses, which limits the possibility of continued dose escalation. Although these findings may pre...

A 56-year-old woman in Helena, Montana, USA, who showed clinical signs of paralysis, received antitoxins to botulinum toxins A, B, and E within 24 hours; nevertheless, symptoms progressed to complete quadriplegia. On day 8, she began moving spontaneously, even though blood tests later showed botulinum toxin type F remained.

To the Editor: In the October 2005 issue of Emerging Infectious Diseases, Song et al. described a fiber-optic, microsphere-based, highdensity array composed of 18 speciesspecific probe microsensors, used to identify biological warfare agents, including Clostridium botulinum (1). Although the researchers used multiple probes for C. botulinum, we doubt that this approach is suitable for this orga...

To the Editor: In the October 2005 issue of Emerging Infectious Diseases, Song et al. described a fiber-optic, microsphere-based, highdensity array composed of 18 speciesspecific probe microsensors, used to identify biological warfare agents, including Clostridium botulinum (1). Although the researchers used multiple probes for C. botulinum, we doubt that this approach is suitable for this orga...