stem cells are self-renewing cells that can be differentiated into other cell types. conventional in vitro models for studying stem cells differentiation are usually preformed in two-dimensional (2d) cultures. the design of three-dimensional (3d) in vitro models which ideally are supposed to mimic the in vivo stem cells microenvironment is potentially useful for inducing stem cell derived tissu...

objective(s):immunosuppressive property of mesenchymal stem cells (mscs) has great attraction in regenerative medicine especially when dealing with tissue damage involving immune reactions. the most attractive tissue sources of human mscs used in clinical applications are bone marrow (bm), adipose tissue (at), and wharton's jelly (wj) of human umbilical cord. the current study has compared...

BACKGROUND: Stem cell therapy in small animal medicineis still in its infancy and few in vitro and in vivo research projectsregarding animal Mesenchymal Stem Cells (MSCs) have beencarried out. On the other hand, Cell tracking is the first step of thecell-based therapies and is essential to recognize cell fate posttransplantation. OBJECTIVES: The aim of this study was toisolate, characterize, an...

Studies on hematopoiesis have focused on the function and composition of human bone marrow stroma. Stroma function gives hematopoietic stem cells the microenvironment appropriate for self-renewal and/or prompt differentiation into hematopoietic progenitor cells, then into terminal specialized cells. Human bone marrow stroma has been dissected into hematopoietic and nonhematopoietic components. ...

introduction: bone marrow (bm) is a known source of mesenchymal stem cells (mscs) that are used for cell therapy. this study attempts to identify if the wharton’s jelly (wj) is a suitable substitute for bm as a source for mscs. materials and methods: a population of human wj and bm stem cells were isolated and incubated with fluorescein conjugated antibodies for five specific msc markers. cell ...

background chronic allograft nephropathy(can)  is one of the most common causes of chronic and end stage renal disease. it  is defined with mainly tubular atrophy and  interstitial fibrosis and no evidence of any other etiology, or functional disorder that caused at least three months after transplantation . control of risk factors (htn,dm,hlp, …) and limiting  usage of calcineurin inhibitors o...

Background Chronic allograft nephropathy(CAN)  is one of the most common causes of chronic and end stage renal disease. It  is defined with Mainly tubular atrophy and  interstitial fibrosis and no evidence of any other etiology, or functional disorder that caused at least three months after transplantation . Control of risk factors (HTN,DM,HLP, …) and limiting  usage of calcineurin inhibitors...

background: bone marrow-derived mesenchymal stem cells (bmmscs) transplantation has been considered as a promising milestone in liver fibrosis treatment. however, low amounts of homing are a major obstacle. we aimed to investigate the role of melatonin pretreatment in bmmsc homing into experimental liver fibrosis. methods: bmmscs were obtained, grown, propagated and preconditioned with 5 µm mel...