The aim of this paper was to present our experience with seven monoclonal antibodies, six of them were applied in immunohistochemistry and immunoblotting of MyHC isoforms in rats and humans, one of them, 6H1 (Lucas et al., 2000), was tested in human muscle sections only. The four antibodies specific to rat MyHC isoforms, BA-D5,SC-71, BF-35,BF-F3 (Schiaffino et al.,1989) reacted as declared both...

To identify novel or previously described mRNAs that are differentially expressed, numerous investigators have used differential display (DD) (3,4). Briefly, DD involves the reverse-transcription (RT) of mRNA to singlestranded cDNA followed by polymerase chain reaction (PCR) amplification. The PCR conditions and primers used generate a sub-population of cDNA fragments corresponding to the 3′ en...