Extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-E) among animals and humans are a public health threat. This study analyzed the occurrence of ESBL-E in high-risk environment companion animal clinic two patients’ households. In an intensive care unit (ICU), rectal swabs from 74 dogs cats, hand staff 298 surfaces were for ESBL-E. Seventeen hospitalized patients (23%) ten (3%) ...

The aim of this study was to elucidate the epidemiology of third generation cephalosporin resistant Samonella isolates from pork of a slaughterhouse in China and the features of transferable elements carrying blaCTX-M genes. One hundred and twenty-six (7.3%) Salmonella isolates were identified; S. Derby and S. Rissen were the most two prevalent serotypes. Among these isolates 20 (15.8%) were re...

OBJECTIVE To determine the molecular characterization of extended-spectrum beta-lactamases (ESBL) isolates from a tertiary center in Saudi Arabia using multiplex polymerase chain reaction (PCR) technique and assess their antibiotic susceptibility pattern. METHODS Prospective study conducted at the Saudi Aramco Dhahran Health Center, Dhahran, Saudi Arabia between April-December 2006. Extended-...

Presence of extended spectrum beta-lactamases (ESBL) in bacteria is a growing health concern of global significance. The local, regional, national, and international epidemiological studies for extended spectrum beta-lactamases-producing Enterobacteriaceae and their encoding genes in foods are still incomplete. The objective of this study was to determine the occurrence of extended spectrum bet...

OBJECTIVES The aims of this study were (i) to detect extended-spectrum β-lactamase (ESBL) genes among 1378 Escherichia coli isolates from defined disease conditions of companion and farm animals and (ii) to determine the localization and organization of ESBL genes. METHODS E. coli isolates from the German resistance monitoring programme GERM-Vet were included in the study. Plasmids were trans...

We investigated the effect of international travel on the gut resistome of 122 healthy travelers from the Netherlands by using a targeted metagenomic approach. Our results confirm high acquisition rates of the extended-spectrum β-lactamase encoding gene blaCTX-M, documenting a rise in prevalence from 9.0% before travel to 33.6% after travel (p<0.001). The prevalence of quinolone resistance enco...

CONTEXT The CTX-M family consists of more than 50 β-lactamases, which are grouped on the basis of sequences into five subtypes including CTX-M-1, CTX-M-2, CTX-M-8, CTX-M-9 and CTX-M-25. OBJECTIVES The current study aimed to detect subtypes of CTX-M extended-spectrum β-lactamases (ESBLs) among ESBL positive Klebsiella isolates from patients in Kashan, Iran. MATERIALS AND METHODS A total of 1...

Only the plasmid from the human E. coli isolate differed in RFLP fingerprint pattern from the other blaCTX-M-2-carrying plasmids. Southern blot hybridization with a blaCTX-M-2 probe revealed a .10 kb EcoRI fragment in the plasmids from the porcine and broiler E. coli isolates and from the Salmonella Virchow CODA-1 isolate. Two EcoRI fragments of 6 and 10 kb were found in the plasmid from the hu...

BACKGROUND Plasmid encoded blaCTX-M enzymes represent an important sub-group of class A beta-lactamases causing the ESBL phenotype which is increasingly found in Enterobacteriaceae including Klebsiella spp. Molecular typing of clinical ESBL-isolates has become more and more important for prevention of the dissemination of ESBL-producers among nosocomial environment. METHODS Multiple displacem...

objective(s):blactx-mand blaper are two genes that encode class a extended-spectrum β-lactamases (esbls) and can be responsible for therapeutic problems. this study was carried out to evaluate the molecular properties of these genes in clinical isolates of enterobacteriaceae by polymerase chain reaction (pcr), restriction digestion and sequencing. materials and methods: during six months, start...