It is well established by in vivo and in vitro studies that dendritic cells (DCs) originate from hematopoietic progenitor cells. However, the presumed intermediate of Birbeck granule (BG)+ Langerhans cells (LCs) has not been detected in cultures derived from bone marrow or peripheral blood progenitor cells (PBPCs), thus contrasting with the data obtained with cord blood. We show here that large...

The geometric dimensions of the copper shell formed by chemical deposition on a spherical polyethylene granule were calculated. It is shown that main factor determining thickness layer initial size granule. processes destruction during thermal expansion polymer are considered. values limit temperatures in which still retains its integrity depending

Mouse spleen contains CD4+, CD8alpha+, and CD4-/CD8alpha- dendritic cells (DCs) in a 2:1:1 ratio. An analysis of 70 surface and cytoplasmic antigens revealed several differences in antigen expression between the 3 subsets. Notably, the Birbeck granule-associated Langerin antigen, as well as CD103 (the mouse homologue of the rat DC marker OX62), were specifically expressed by the CD8alpha+ DC su...

Dendritic cells and their precursors express PPAR-gamma, whose stimulation has inhibitory effects on the maturation and function of dendritic cells in vivo. Dendritic cells can differentiate in vitro from CD133+ progenitors; the influence of PPAR-gamma stimulation on this process is unknown. We have addressed the effect of PPAR-gamma agonist rosiglitazone, at a concentration as used in clinics,...

Human dendritic cells (DC) can now be generated in vitro in large numbers by culturing CD34+ hematopoietic progenitors in presence of GM-CSF+TNF alpha for 12 d. The present study demonstrates that cord blood CD34+ HPC indeed differentiate along two independent DC pathways. At early time points (day 5-7) during the culture, two subsets of DC precursors identified by the exclusive expression of C...

objective(s) it is well known that prenatal stresses (ps) induce a variety of neurobiological and behavioral alterations, some of them involving the hippocampal formation. this study aimed to determine whether restraint stress influences the neuronal volume and number of granule cells in the hippocampus of adult rat offspring. materials and methods ten wistar pregnant rats were randomly divided...

background: dense granule antigens (gra antigens) of toxoplasma gondii induce strong antibody response in humans and are considered as useful diagnostic antigens. previous studies reported expression of amino terminal gra8 protein in fusion with large tags such as glutathione-s-transferase. the present study aimed to produce soluble full length immunogenic gra8 in bacteria. methods: gra8 comple...

Granulation is a process of monoparticle attachment with particular mechanism to form bigger and compact particle which called granule. application has been used on many industries like pharmacy agriculture industries. Research about granulation done continuously get predictive models for various cases. The model only applicable the specific material in this research expected be useful optimize...

Objective(s) It is well known that prenatal stresses (PS) induce a variety of neurobiological and behavioral alterations, some of them involving the hippocampal formation. This study aimed to determine whether restraint stress influences the neuronal volume and number of granule cells in the hippocampus of adult rat offspring. Materials and Methods Ten Wistar pregnant rats were randomly divi...

In spite of the existing reports on behavioural and biochemical changes related to the cerebellum due to noise stress, not much is known about the effect of noise stress on the neuronal changes in the cerebellum. The present study aims at investigating the effects from one week noise exposure on granule cell number and Purkinje cell volume within the neonate rat cerebellum.15-day-old male Wista...