We report a biofunctionalization strategy for the assembly of catalytically active enzymes within a completely packaged bioMEMS device, through the programmed generation of electrical signals at spatially and temporally defined sites. The enzyme of a bacterial metabolic pathway, S-adenosylhomocysteine nucleosidase (Pfs), is genetically fused with a pentatyrosine "pro-tag" at its C-terminus. Sig...

Owing to larger surface area in micro/nanoelectromechanical systems (MEMS/NEMS), surface forces such as adhesion, friction, and meniscus and viscous drag forces become large when compared with inertial and electromagnetic forces. There is a need to develop lubricants and identify lubrication methods that are suitable for MEMS/NEMS. For BioMEMS/BioNEMS, adhesion between biological molecular laye...

Recent advances in the micromachining of poly(p-xylylenes), commercially known as Parylenes, have enabled the development of novel structures and devices for microelectromechanical systems (MEMS). In particular, Parylene C (poly[chloro-p-xylylene]) has been explored extensively for biomedical applications of MEMS given its compatibility with micromachining processes, proven biocompatibility, an...

Many microfluidic applications require the mixing of reagents, but efficient mixing in these laminar (i.e., low Reynolds number) systems is typically difficult. Instead of using external fields, miniature stirrers, complex geometries, and/or relatively long channels, we demonstrate the merits of flow rate time dependency mixing in a simple “--II-” channel confluence. By means of computational f...

Cost-effective/sensitive/selective/reliable detectors for firstresponders Point-of-care/clinical applications for pathogen/virus/threat detection and identification Technological challenges for rapid/early/specific sensitive detection Role of nanotechnology and system miniaturization; MEMS and bioMEMS Use of biochip technology; microand nanofluidics Bioinformatics for biodefense Reducing false ...

Cost-effective/sensitive/selective/reliable detectors for firstresponders Point-of-care/clinical applications for pathogen/virus/threat detection and identification Technological challenges for rapid/early/specific sensitive detection Role of nanotechnology and system miniaturization; MEMS and bioMEMS Use of biochip technology; microand nanofluidics Bioinformatics for biodefense Reducing false ...