Multicolor bioimaging can be referred to as the imaging method that non-invasively visualizes biological processes using fluorophores.

Highly amino-functionalized fluorescent carbon nanoparticles (CNPs) were fabricated by hydrothermal carbonization of chitosan at a mild temperature. They were applied to bioimaging of human lung adenocarcinoma A549 cells, showing low cytotoxicity and excellent biocompatibility.

The afterglow of phosphorescent compounds can be distinguished from background fluorescence and scattered light by a time-resolved observation, which is a beneficial property for bioimaging. Phosphorescence emission accompanies spin-forbidden transitions from an excited singlet state through an excited triplet state to a ground singlet state. Since these intersystem crossings are facilitated us...

Fluorescent probes, which allow visualization of cations such as Ca(2+), Zn(2+) etc., small biomolecules such as nitric oxide (NO) or enzyme activities in living cells by means of fluorescence microscopy, have become indispensable tools for clarifying functions in biological systems. This review deals with the general principles for the design of bioimaging fluorescent probes by modulating the ...

Here, we report a straightforward synthesis process to produce colloidal Eu-activated nanophosphors (NPs) for use as bioimaging probes. In this procedure, poly(ethylene glycol) serves as a high-boiling point solvent allowing for nanoscale particle formation as well as a convenient medium for solvent exchange and subsequent surface modification. The La(OH)3:Eu 3+ NPs produced by this process wer...

Polyacrylic acid (PAA) modified NaYF4:Gd/Yb/Er upconversion nanorods (denoted as PAA-UCNRs) are demonstrated for tri-modal upconversion (UC) optical, computed X-ray tomography (CT), and magnetic resonance imaging (MRI). The hydrophilic PAA-UCNRs were obtained from hydrophobic oleic acid (OA) capped UCNRs (denoted as OA-UCNRs) using a ligand exchange method. The as-prepared UCNRs with a hexagona...

Presently, EPR and fluorescent biosensors are sole choice of in vivo bioimaging of NO and accurate measurement still remains a problem. We propose different NO biosensors as potent imaging contrast agents using multimodal bioimaging techniques of MRI, EPR and fluorescent imaging with their limitations and quantitative limits. Our focus in this paper is on different characteristics of known bios...

Herein, a label-free multiplex photoluminescent silicon nanoprobe (PLSN-probe) is introduced as a potential substitute for quantum dots (QDs) in bioimaging. An inherently non-photoluminescent silicon substrate is altered to create the PLSN-probe, to overcome the major drawbacks of presently available QDs. Additionally, crystallinity alterations of the multiplane crystalline PLSN-probes lead to ...

Hydrotalcite-like compounds are two-dimensional inorganic nanolayers also known as clay minerals or anionic clays or layered double hydroxides/layered hydroxy salts, and have emerged as a single type of material with numerous biomedical applications, such as drug delivery, gene delivery, cosmetics, and biosensing. Inorganic nanolayers are promising materials due to their fascinating properties,...

The ideal fluorescent probe for bioimaging is bright, absorbs at long wavelengths and can be implemented flexibly in living cells and in vivo. However, the design of synthetic fluorophores that combine all of these properties has proved to be extremely difficult. Here, we introduce a biocompatible near-infrared silicon-rhodamine probe that can be coupled specifically to proteins using different...