Rational design for a new spiroxindoles, combined with benzimidazole scaffold to identify murine double minute two (MDM2) inhibitor was synthesized and characterized. The desired spiroxindoles were achieved via [3+2] cycloaddition reaction approach which afforded the cycloadducts four asymmetric centers separated in an excellent regioselective diastereoselective compound. subjected set of bioch...

Objective: To determine the phytochemical constituents, antioxidant and anticancer activities of Macranga peltata leaf extracts on A549 P 51 human lung cancer cell lines.
 Methods: Leaf sample was subjected to soxhlet extraction method screening done using different biochemical tests. The activity tested 2,2–diphenyl-1-picrylhydrazyl, ferric ion reducing power assay 2,2–azino bis 3–ethyl b...

A method for the determination of a-1-fetoprotein (AFP) in human serum by using a horseradish peroxidase (HRP) label in an enzymelinked immunosorbent assay (ELISA) is proposed. The method is based on the electrochemical determination of enzymatic reaction product with differential pulse voltammetry at a gold disk electrode. The assay consists of two successive steps. The ®rst step is a conventi...

A sensitive biochemical assay of viral reverse transcriptase (RT) was developed that is useful for both the detection and quantitation of human immunodeficiency virus (HIV), the agent responsible for acquired immune deficiency syndrome in humans. This assay gave a 20- to 40-fold increase in enzyme activity over the current method used for RT detection of HIV. The test is based on a previous bio...

Objective: The aims of the present study were to screen actinobacteria with high potential ability produce secondary metabolites that have inhibitory activity against plant pathogenic fungi, Magnaporthe oryzae. Production was analysis by thin-layer chromatography and bioautography assay.
 Methods: Screening selection Streptomyces sp. morphological, cultural, physiological, biochemical char...

Collagen quantification has long been relevant to biomedical research and clinical practice to characterize tissues and determine disease states. The hydroxyproline assay, while a broadly employed method of quantifying collagen, uses perchloric acid to dissolve Ehrlich's reagent. Since perchloric acid poses occupational safety hazards and high costs, in this study, a new hydroxyproline assay wa...

Our study involves development of fluorescent cell-based diagnostic assay as a new approach in high-throughput screening method. This highly sensitive optical assay operates similarly to e-noses and e-tongues which combine semi-specific sensors and multivariate data analysis for monitoring biochemical processes. The optical assay consists of a mixture of environmental-sensitive fluorescent dyes...