NCBI’s PubChem BioAssay database (1-5) (http://pubchem.ncbi.nlm.nih.gov) is a public repository for archiving biological tests of small molecules and siRNA reagents. Small molecule bioactivity data contained in the BioAssay database consist of information generated through high-throughput screening experiments, medicinal chemistry studies, chemical biology research, as well as literature curati...

The repellents N,N-diethyl-3-methylbenzamide (deet) and racemic 2-methylpiperidinyl-3-cyclohexene-1-carboxamide (AI3-37220) were evaluated using two different laboratory bioassays to determine their relative effectiveness against host-seeking nymphs of the blacklegged tick, Ixodes scapularis Say, and the lone star tick, Amblyomma americanum (L.). In a petri dish bioassay, ticks were released wi...

The aim of this study was to develop and validate a simple, sensitive, precise and cost-effective one-level agar diffusion (5+1) bioassay for estimation of potency and bioactivity of Levofloxacin in pharmaceutical preparation which has not yet been reported in any pharmacopoeia. Among 16 microbial strains, Bacillus pumilus ATCC-14884 was selected as the most significant strain against Levofloxa...

INTRODUCTION Auxins are hormones that regulate plant growth and development. To accurately quantify the low levels of auxins present in plant and soil samples, sensitive detection methods are needed. In this study, the design and construction of two different whole cell auxin bioassays is illustrated. Both use the auxin responsive element HpaA as an input module but differ in output module. The...

In this study, we used two different types of bioassay, a contact filter paper toxicity bioassay and a soil toxicity bioassay, to compare the acute toxicity of twenty-four insecticides belonging to six chemical categories on earthworm species, Eisenia fetida. Results of the contact filter paper toxicity bioassay indicated that neonicotinoids were super toxic to E. fetida (48 h-LC(50) value rang...

This work proposes a procedure to estimate the measurement uncertainty for the quantification of cefazolin by high-performance liquid chromatography (HPLC) and microbiological assay (bioassay). HPLC method was performed according to United States Pharmacopeia (USP) and bioassay according to Brazilian Pharmacopeia (FB). The quantification of cefazolin by high-performance liquid chromatography wa...

PubChem's BioAssay database (http://pubchem.ncbi.nlm.nih.gov) is a public repository for archiving biological tests of small molecules generated through high-throughput screening experiments, medicinal chemistry studies, chemical biology research and drug discovery programs. In addition, the BioAssay database contains data from high-throughput RNA interference screening aimed at identifying cri...