Seven mouse hybridoma cell lines producing monoclonal antibodies (MAb) against an encephalitogenic strain of bovine herpesvirus type 1.3 (BHV-1.3) were established. The clones producing MAb were selected to be specific for BHV-1.3 by enzyme-linked immunosorbent assay. Only L1B neutralized virus without complement. With the addition of complement, five of the MAb neutralized BHV-1.3 but not the ...

Bovine herpesvirus 1 (BHV-1) infection is widespread and causes a variety of diseases. Although similar in many respects to the human immune response to human herpesvirus 1, the differences in the bovine virus proteins, immune system components and strategies, physiology, and lifestyle mean the bovine immune response to BHV-1 is unique. The innate immune system initially responds to infection, ...

A total of 255 sera were collected from unvaccinated crossbred (174) and indigenous (81) cattle and buffalo having history of respiratory and reproductive disorders. All the sera sample were subjected to indirect ELISA and passive haemagglutination (PHA) test for the detection of BHV-1 antibodies. Indirect ELISA and PHA test detected significantly (P<0.01) varied prevalence of BHV-1 antibody i....

bvdv and bhv-1 are considered as worldwide, complicated and economically significant infections associated with a range of clinical syndromes in cattle. as clinical syndromes such as pneumonia, diarrhea, abortion and reproductive losses were repeatedly reported in dairy herds in suburbs of arak, this study was planned to determine the prevalence of antibodies to bvdv and bhv-1 in industrial dai...

The UL3.5 gene is positionally conserved but highly variable in size and sequence in different members of the Alphaherpesvirinae and is absent from herpes simplex virus genomes. We have shown previously that the pseudorabies virus (PrV) UL3.5 gene encodes a nonstructural protein which is required for secondary envelopment of intracytoplasmic virus particles in the trans-Golgi region. In the abs...

The interaction of bovine herpesvirus 1 (BHV-1) with the BHV-1 glycoprotein IV (gIV)-expressing cell line D1-1 was examined by radiolabelled virus adsorption assays, in situ autoradiography and electron microscopy. Adsorption of radiolabelled BHV-1 to D1-1 cells was similar to that observed in control cell lines but in situ radiography revealed that virus moved to the nucleus of control but not...

Bovine herpes viruses belong to the family Herpesviridae. Bovine herpes virus 1 (BHV-1) is of world wide distribution and causes a wide range of diseases in cattle namely respiratory disease, keratoconjunctivitis and reproductive diseases including infectious pustular vulvovaginitis (IPV) in cows, infectious pustular balonoposthitis (IPB) in bulls and abortions. DNA studies of BHV-1 have reveal...

Infectious bovine rhinotracheitis (IBR) is caused by Bovine Herpes Virus-1(BHV-1), a member of the genus Varicellovirus in the sub-family Alphaherpesvirinae, family Herpesviridae and order Herpesvirales. Virions are enveloped and variably sized (approximately120–250 nm in diameter), containing an icosahedral nucleocapsid composed of 162 capsomers. Genome is linear double-stranded DNA, 125–290 k...

Acute infection of cattle with bovine herpesvirus 1 (BHV-1) represses cell-mediated immunity, which can lead to secondary bacterial infections. Since BHV-1 can induce apoptosis of cultured lymphocytes, we hypothesized that these virus-host interactions occur in cattle. To test this hypothesis, we analyzed lymph nodes and peripheral blood mononuclear cells (PBMC) after calves were infected with ...

In the present study, we report for the first time on the detection of bovine herpesvirus type 1 (BHV-1) in whole-blood samples derived from naturally infected cattle. Sensitive PCR assays specific for glycoprotein B (gB), gC, and gE of BHV-1 allow the detection of one BHV-1 DNA copy in 10(5) to 10(7) peripheral blood leukocytes (PBLs). The incidence of BHV-1-positive PBLs in naturally infected...