One drawback when using non-radioactive labelling has been that there is no easy test for the degree of incorporation of nucleotide analogues. Here we show that biotinylated DNA-fragments have a lower mobility than non-biotinylated fragments during agarose gel electrophoresis, and that this gives a good indication of the degree of end-labelling of DNA-fragments. This feature can be exemplified ...

BACKGROUND To isolate over-secretors, we subjected to saturation mutagenesis, a strain of P.pastoris exporting E. coli alkaline phosphatase (EAP) fused to the secretory domain of the yeast α factor pheromone through cellular PHO1/KEX2 secretory processing signals as the α-sec-EAP reporter protein. Direct chromogenic staining for α-sec-EAP activity is non-specific as its NBT/BCIP substrate cross...

A laboratory study was carried out on 44 sera of typhoid patients (age: 15-45 years, 24 females and 20 males) with positive blood culture for S. typhi, 41 sera non typhoid febrile patients (age: 16-51 years, 17 females & 24 males) with negative blood culture and negative Widal test, 136 sera of healthy persons as normal controls (age: 20-49 years, 95 females & 41 males). The basic principle of ...

AIM: To compare the clinicopathological features of pancreatic intraepithelial neoplasias (PanINs) and intraductal papillary mucinous neoplasms (IPMNs), and to investigate the role of hsa-miR-96 and hsa-miR-217 in these two lesions. Methods: Formalin-fixed paraffin-embedded pancreatic specimens were selected in this study, including 58 cases of pancreatic intraepithelial neoplasias (PanINs), 45...

Vajner L. , V. Konrádová, J . Uhl ík , J . Zocová: Mucin Histochemistry of Tracheal Goblet Cells after Oral Administration of Ambroxol. Acta Vet. Brno 2001, 70: 9-13. Previous studies on the effect of various mucolytic drugs on the tracheal epithelium ultrastructure revealed ambroxol as the most harmful one. To complete these studies, we decided to evaluate the effect of ambroxol on the glycoco...

Recently it was discovered that the bacteriophage M13 contains in its protein III gene, tandem repeats of a 15-bp motif that can recognize a family of hypervariable minisatellites in vertebrate DNA (1). Southern blots of human DNA digested with Hae III and probed with 32p_iaDeled M13 revealed complex individual-specific DNA fingerprints, similar to those described by Jeffreys et al (2) with pro...

Virulence determinants are inclined to be located on the bacterial surface or secreted into the milieu where they can interact with the host. A system that isolates defined mutations in secreted bacterial products is an invaluable tool for identifying virulence genes. One such system is the phoA gene-fusion approach that takes advantage of the fact that the normally periplasmic bacterial protei...