Alkali-pretreated sugarcane bagasse fiber was subjected to fed-batch simultaneous saccharification and fermentation (SSF) with a prehydrolysis process to increase the solids loading and produce a high concentration of ethanol. The hydrolysis medium and yeast feeding modes were investigated to determine suitable conditions for high sugar yield and ethanol production. Batch addition resulted in a...

Porphyridium cultures grown on either nitrate or ammonium as the nitrogen source showed similar patterns of growth and cell wall polysaccharide production. The effect of nitrogen on growth and cell wall polysaccharide production was studied by applying three regimens of supply: batch mode, in which nitrate was supplied at the beginning of the experiment and became depleted at day 6; continual m...

Background: The increase of the protein expression via ribosomal manipulation is one of the suggested cellular mechanisms involved in EnBase fed-batch mode of cultivation. However, this system has not been implemented for cytotoxic proteins.Objectives: Here, the expression pattern of α-Luffin, a ribosome inactivation protein (RIP) with an innate toxicity,...

the performance of a plug flow electrocoagulation reactor for the treatment of cattle abattoir(slaughterhouse) wastewater was investigated. a cylindrical iron reactor was operated as a cathode at arecycling batch mode while three iron rods located at the center of the reactor were used as an anode. theeffects of different parameters, including the current density, the supporting electrolyte dos...

During the pretreatment of cellulosic biomass for subsequent microbial or enzymatic processing, the fiber reactivity typically increases with increasing severity but so does sugar degradation. Experimental results with sugarcane bagasse show that this tradeoff can be mitigated substantially by pretreatment in a flow-through (FT) mode. A model that incorporates both kinetics and mass transfer wa...

Retroviral pseudotype vector, derived from the murine leukaemia virus carrying the HIV-1 envelop protein MLV (HIV-1) were produced using a integrated, continuous cultivation and harvest process. A 200ml fix bed reactor was used to cultivate the anchorage dependent packaging cell line on macro-porous carriers until the maximum glucose uptake was reached. After starting the cultivation in batch m...

Computational grid is a new paradigm in parallel and distributed computing systems for realizing a virtual supercomputer over idle resources available in a wide area network like the Internet. Computational Grids are characterized for exploiting highly heterogeneous resources; so, one of the main concerns in developing computational grids is how to effectively map tasks onto heterogeneous resou...

The results and conclusions of this analysis are those of the authors and do not necessarily reflect concurrence by the World Bank. We thank Debabrata Das for superb research assistance. 1 For example, recent studies indicate that about half of the productivity growth in U.S. 1 manufacturing over the 1980s is accounted for by the reallocation of resources from less productive to more productive...