An ethyl anthranilate azopigment of bilirubin conjugated to fi-D-monoglucoside was isolated from dog gall-bladder bile. Glucose was cleaved from the azopigment by treatment with IJ-glucosidase and f6-glucuronidase. Mild alkaline hydrolysis of the compound by sodium methoxide yielded two kinds of compounds, water-soluble and organic-soluble. The former were shown, by enzymic analysis, t.l.c., nu...