Aiming to develop a DNA marker specific for Bacillus anthracis and able to discriminate this species from Bacillus cereus, Bacillus thuringiensis, and Bacillus mycoides, we applied the randomly amplified polymorphic DNA (RAPD) fingerprinting technique to a collection of 101 strains of the genus Bacillus, including 61 strains of the B. cereus group. An 838-bp RAPD marker (SG-850) specific for B....

The nucleotide sequences of the 3' end of the 16S rDNA and the 16S-23S internal transcribed spacer (ITS) of 40 Bacillaceae species were determined. These included 21 Bacillus, 9 Paenibacillus, 6 Brevibacillus, 2 Geobacillus, 1 Marinibacillus and 1 Virgibacillus species. Comparative sequence analysis of a 220 bp region covering a highly conserved 150 bp sequence located at the 3' end of the 16S ...

Microbial culturomics, which investigates microbial diversity by combining diversified culture conditions, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and 16S rDNA identification, allowed to identify five new species within the Bacillus genus. Bacillus massiliglaciei strain Marseille-P2600T, Bacillus mediterraneensis strain Marseille-P2384T, Bacillus massilinige...

0.094 ----TSB 37°C Baciillus sp. Clone 35 23 0.047 ----TSB 37°C Bacillaceae bacterium C22 21 1.7 ----BA 37°C Bacillus cereus 43 0.75 ----BA 30°C 38 0.5 ----TSB 30°C Bacillus fusiformis 14 1.5 ----BA 37°C Bacillus aquimaris 45 1.2 CXM30 4.25mm BA 30°C Bacillus pumilus 48 3.5 ----R2A 37°C Bacillus megaterium 32 0.064 ----BA 30°C 42 0.75 ----TSB 37°C 19 0.25 ----TSB 30°C 18 0.38 CXM30 1.75mm LB 37°C

A bronchopneumonia of calves in the early months of life is described and its etiology associated definitely with a minute bacillus, Bacillus actinoides. Bacillus pyogenes, Bacillus bovisepticus, and, less frequently, staphylococci and streptococci may appear later in the affected lungs. Subcutaneous injections of cultures of Bacillus actinoides produce large indurations ending in necrosis. Sim...

The complete sequencing and annotation of the genomes of industrially-important Bacillus species has enhanced our understanding of their properties, and allowed advances in genetic manipulations in other Bacillus species. Post-genomic studies require simple and highly efficient tools to enable genetic manipulation. Here, we summarize the recent progress in genetic engineering strategies for Bac...

Bacillus spp. are Gram-positive spore-forming bacteria used commercially as probiotics in the larviculture of fish. Bacillus spp. can act positively on cultured organisms by enhancing their survival and growth, stimulating their digestive and immune systems, and improving water quality. In this present study, grass carp larvae were fed on Artemia urmiana, Artemia fransiscana and Artemia parthen...

There are many studies on the effects of probiotics on performance of broiler chickens, but none of them has evaluated the metabolizable energy (ME) equivalency values of probiotics. The aim of this study was to determine the metabolizable energy equivalency value of Bacillus subtilis spore and its potential for decreasing feed ME content and cost. One hundred seventy-six day-old male broilers ...