1. The sera of twenty-six individuals who carried tetanus bacilli in their digestive tracts all contained appreciable amounts of antitoxin. 2. The sera of thirty individuals in whose stools no tetanus-like organisms were found were, with two exceptions, free from tetanus antitoxin. 3. Although we have been unable to measure accurately the antitoxin content of these human carriers of tetanus bac...

As part of a series of investigations at Baba Baghi Leprosarium in lran, 44 long-treated leprosy patients were selected for our study. Samples of early morning sputum were obtained from each patient, examined by microscopy for acid-fast bacilli (AFB), and cultured for tubercle bacilli. These tests were negative, but the polymerase chain reaction (PCR) for an insertion sequence believed to b...

To develop a method for staining acid fast bacilli which excluded highly toxic phenol from the staining solution. A lipophilic agent, a liquid organic detergent, LOC High Studs, distributed by Amway, was substituted. The acid fast bacilli stained red; nuclei, cytoplasm, and cytoplasmic elements stained blue on a clear background. These results compare very favourably with acid fast bacilli stai...

When monocytes derived from normal guinea pigs or rabbits were infected with tubercle bacilli and cultivated in vitro, the bacilli multiplied abundantly within the cytoplasm of these cells. By contrast, intracellular multiplication of the bacilli was retarded or completely inhibited within the monocytes of rabbits or guinea pigs vaccinated with BCG. This inhibition of growth was observed with b...

1. Tubercle bacilli when suspended in. distilled water, normal salt solution, bouillon and milk, are destroyed at 60 degrees C. in 15 to 20 minutes. The larger number are destroyed in 5 to 10 minutes. 2. When tubercle bacilli are suspended in milk, the pellicle which forms during the exposure at 60 degrees C. may contain living bacilli after 60 minutes.

Fiberoptic bronchoscopy was performed in 30 cases of suspected pulmonary tuberculosis. Sputum was negative for tubercle bacilli in all the patients, but in specimens obtained at bronchoscopy tubercle bacilli were found in 86.6%. By postbronchoscopy sputum smear examination tubercle bacilli were detected in 73.3% of the cases.

At intervals from 2 to 11 weeks after normal rabbits had inhaled small numbers of virulent bovine tubercle bacilli as separated cells in droplet nuclei, groups of these animals received a single exposure to reinfection during which each animal inhaled about 20,000 separated bacilli. Normal control rabbits which inhaled this large number of bacilli died within 4 weeks thereafter. Their deaths we...