Five new pesticides were evaluated for their effectiveness in the control of Tuta absoluta (Meyrick), (Lepidoptera : Gelechiidae) on tomato plant. The effect of pesticides against egg and larvae were estimated after 2, 5 and 7 days from application. The highest reduction percentage of eggs of Tuta absoluta during two successive seasons achieved by Emacit (81.25 and 74.5) % respectively after 7 ...

Azadirachta indica (neem) is the only source of azadirachtin, which is known for its insecticide activity. Melia azedarach is a related species of A. indica, widely distributed in the south of China. In this study, the leaf transcriptomes of these two Meliaceae plants were sequenced. More than 40 million clean reads were generated from each library. About 80 % of A. indica reads were mapped to ...

Azadirachtin, derived from the neem tree, Azadirachta indica A. Juss (Sapindales: Meliaceae), seems promising for use in integrated pest management programs to control a variety of pest species. A commercial formulation of azadirachtin, Align, has been evaluated against different developmental stages of the European grape berry moth, Lobesia botrana Denis and Schiffermüller (Lepidoptera: Tortri...

Mitochondria have been shown to play an important role in apoptosis using mammalian cell lines. However, this seems not to be the case in Drosophila, an insect model organism; thus more in-depth studies of insect cell apoptosis are necessary. In the present study, mitochondrial involvement during azadirachtin- and camptothecin-induced apoptosis in Spodoptera frugiperda Sf9 cells (isolated from ...

The following stress factors were tested for their effect on the susceptibility of Plutella xylostella (L.) to Bacillus thuringiensis Berliner: (a) exposure to low doses of the synthetic pyrethroid cypermethrin, (b) treatment with low doses of the secondary plant compound, azadirachtin and (c) development on partially resistant cabbage cultivars. B. thuringiensis-related mortality of cypermethr...

The present study describes the major content and antimicrobial activity of Azadirachta indica cell culture extracts. Major constituents of cell culture extracts were evaluated by HPLC and chromatographic analysis revealed that highest concentrations of azadirachtin (0.0069 % dry wt) synthesised by multiple shoot cultures. The results showed that multiple shoot cultures synthesised ~17-fold mor...

Neem (Azadirachta indica A. Juss) is one of the most versatile tropical evergreen tree species known in India since the Vedic period (1500 BC-600 BC). Neem tree is a rich source of limonoids, having a wide spectrum of activity against insect pests and microbial pathogens. Complex tetranortriterpenoids such as azadirachtin, salanin and nimbin are the major active principles isolated from neem se...

Present report is the first direct evidence of azadirachtin production in androgenic haploid cultures of Azadirachta indica, a woody medicinal tree. Anther cultures at early-late-uninucleate stage of microspores were established on MS medium with BAP (5 μM), 2,4-D (1 μM) and NAA (1 μM) containing 12% sucrose. The calli, induced, were further multiplied on 2,4-D and Kinetin media. Shoots, differ...

Melanaphis sacchari (Zehntner) is a major pest of sorghum, Sorghum bicolor (L.) Moench, in the United States and neighboring Caribbean countries including Haiti. Laboratory, greenhouse, field experiments were conducted to determine effects biological insecticides on M. infesting sorghum. Azadirachtin, pyrethrins, Beauveria bassiana strain GHA, Isaria fumosorosea Apopka 97, Chromobacterium subts...

in this study the effect of different concentrations of some abiotic elicitors (salicylic acid, jasmonic acid, cadmium chloride and sodium chloride) with different incubation times (3, 6, 9 and 12) on azadiractin production and cell growth in the cell culture of the iranian native neem were investigated. cell growth rate was measured by changes in dry cell weight and it was used as a basis for ...