We investigated the effect of the cyclic AMP-protein kinase A (PKA) signalling pathway on renal Na(+),K(+)-ATPase and ouabain-sensitive H(+),K(+)-ATPase. Male Wistar rats were anaesthetized and catheter was inserted through the femoral artery into the abdominal aorta proximally to the renal arteries for infusion of the investigated substances. Na(+),K(+)-ATPase activity was measured in the pres...

Introduction Phosphorus (P) deficiency hinders cotton ( Gossypium hirustum L.) growth and development, seriously affecting lint yield fiber quality. However, it is still unclear how P fertilizer affects length. Methods Therefore, a two-year (2019-2020) pool-culture experiment was conducted using the split-plot design, with two cultivars (CCRI-79; low-P tolerant SCRC-28; sensitive) as main plot....

Exposure to acidic and alkaline pHs results in an ionic imbalance. Cellular responses involved osmoregulation silver catfish exposed different (5.5, 7.5, 9.0) for 24 h were evaluated. The gills kidney collected measure Na+/K+-ATPase (NKA) H+-ATPase (V-ATPase) activities evaluate the expression of ion transporter-related genes: NKA (atp1a1), H+-ATPases (atp6v0a1b, atp6v0a2a, atp6v0a2b), Na+/H+ a...

wilson disease is a metabolic disorder with an autosomal recessive genetic pattern and occurs in 1-4 of every 100000 individuals. inactivation of the atp7b gene leads to accumulation of the toxic copper to liver and brain causing hepatic and neurological complication. therefore, most patients suffer from chronic hepatic inflammation and central nervous system disorder. nowadays, up to 500 mutat...

In many tissues, the level of cytoplasmic calcium mediates cell function. Since cytoplasmic calcium is often maintained at a low level by active calcium extrusion, we examined whether calcium-stimulated ATPase is present in the rabbit ciliary epithelium. A technique was developed to measure calcium-stimulated ATPase in a partially enriched plasma membrane preparation. The enhancement of Na,K-AT...

The present study was conducted to find the effect of three heavy metals, Ag, Hg and Pb on the expression level of a gene encoding plasma membrane H+-ATPase in Aeluropus littoralis. The experiment was laid out in a completely random design with three replications. The expression of the main gene was normalized to the expression of the housekeeping gene actin. Two 259 and 187 bp fragments were a...

Oliveira-Souza, Maria, Raif Musa-Aziz, Gerhard Malnic, and Margarida de Mello Aires. Arginine vasopressin stimulates H ATPase in MDCK cells via V1 (cell Ca ) and V2 (cAMP) receptors. Am J Physiol Renal Physiol 286: F402–F408, 2004. First published September 9, 2003; 10.1152/ajprenal.00121.2003.—The effect of arginine vasopressin (AVP) and/or atrial natriuretic peptide (ANP) on the regulation of...

Previous studies in kidney, heart, and liver cells have demonstrated that dexamethasone regulates the expression of Na-K-ATPase. In the lungs, Na-K-ATPase has been reported in alveolar epithelial type II (ATII) cells and is thought to participate in active Na+ transport and lung edema clearance. The aim of this study was to determine whether Na-K-ATPase would be regulated by dexamethasone in cu...

huntington disease (hd) is a genetically dominant condition caused by expanded cag repeats which code for glutamine in the hd gene product, huntingtin. huntingtin is expressed in almost all tissues, so abnormalities outside the brain can also be expected. involvement of nuclei and mitochondria in hd pathophysiology has been suggested. in fact, mitochondrial dysfunction is reported in brains of ...

the ultrastructure of the cells, na+, k+-atpase activity and immunolocalization were examined in the barnchial chamber of libellula lydia (drury, 1773) larvae. na+,k+-atpase activity and localization were performed through biochemical techniques and immunofluorescence light microscopy using a mouse monoclonal antibody iggα5, respectively. the branchial chamber possesses six pair gills lamellae ...