The isolation of a single DNA molecule for cloning is technically difficult, and the subsequent screening of colonies for recombinant DNA clones is time consuming. Ion pair-reversed phase HPLC (IP-RP-HPLC) analysis of nucleic acids improves the resolution and isolation of PCR products to be cloned. We demonstrate that PCR products analyzed and collected using the IP-RP-HPLC approach (WAVE DNA F...

Abstract Background A new, sensitive, suitable, clear, accurate, and robust reversed-phase high-performance liquid chromatography (RP-HPLC) method for the determination of brexpiprazole in bulk drug tablet formulation was developed validated this research. Surface methodology used to optimize data, with a three-level Box-Behnken design. Methanol concentration mobile phase, flow rate, pH were ch...

Objectives: To perform stability indicating analytical method development and validation of Lafutidine in tablet dosage form by reverse phase HPLC method. Materials and Methods: The method was developed employing isocratic RP-HPLC and validated for assay of Lafutidine in tablet dosage form. The method employs the use of a UV detector. Results: The developed assay method was found to be accurate...

We present a rapid, sensitive, and automated HPLC method with direct resolution of l-folinic acid (l-FA), d-folinic acid (d-FA), and 5-methyltetrahydrofolate (5MTHF) from plasma samples. Plasma (500 microL) is first extracted on solid phase (RP-18 cartridge). The dI-FA peak is collected on-line from a reversed-phase column (RP-8, 119 x 2 mm, 4 microns: HPLC 1) and then automatically loaded onto...

losartan potassium, valsartan , telmisartan and irbesartan are angiotensin-ii-receptor antagonists (ara ii) group which used in treatment of hypertension alone or in combination with other drugs mainly hydrochlorothiazide. rp- hplc method was developed for the assay of three angiotensin-ii-receptor antagonists (ara-iis) in presence of hydrochlorothiazide. the method was performed by reversed ph...