Four distinct isozymes of aspartate-alpha-ketoglutarate transaminase in a spinach (Spinacia oleracea L.) leaf extract were separated by starch gel electrophoresis. Of the total aspartate-alpha-ketoglutarate transaminase activity, approximately 45% was represented by the chloroplast isozyme, 26% by the cytosol isozyme, 19% by the mitochondrial isozyme, and 3 to 10% by the peroxisomal isozyme. Th...

The characteristics of liver damage associated with the use of diclofenac, a popular nonsteroidal anti-inflammatory drug, were investigated by reviewing adverse drug reaction reports for Australia. Twenty six patients were reported for whom diclofenac was the sole suspected drug cause of their liver damage. The average age of the patients was 64 years (range 37-84 years); 19 (70%) were women. T...

Methods: The effect of Abana pretreatment (75 mg /100 g ) for a period of 60 days on isoproterenol (20 mg/100 g s.c. twice at an interval of 24 hrs) induced lipid peroxidation was studied in rats. Marker enzymes levels such as creatine kinase, lactate dehydrogenase, alanine transaminase and aspartate transaminase were assessed in serum and heart homogenate. Glutathione content and lipid peroxid...

The reactions of two analogues of 4-aminobutyrate, namely 4-aminohex-5-ynoate and 4-aminohex-5-enoate, with three transaminases were studied. Three pure enzymes were used, aminobutyrate transaminase (EC 2.6.1.19), ornithine transaminase (EC 2.6.1.13) and aspartate transaminase (EC 2.6.1.1), and the course of the reactions was studied by observing changes in the absorption spectrum of the bound ...

Clinical Enzymology Enzymes nomenclature, classification, structure and specificity Enzymes, isoenzymes and their relevance in diagnosis Regulation of enzyme levels in serum and plasma Selection of enzyme tests Measurement of isoenzymes and isoforms Some important enzymes in clinical practice Aspartate transaminase (AST) and Alanine transaminase (ALT) Creatine Kinase (CK) Lactate dehydrogenase ...

Blood samples were collected from all study-group subjects for biochemical parameters assays before and after 15 days of vitamin C supplements. The study clearly showed that giving vitamin C supple ments protects liver damage in vineyard crop sprayers, which results in decreased aspartate transaminase and alanine transaminase activity. In addition to its antioxidant activity, vitamin C is known...