Experiments were conducted with intact rat hepatocytes to identify inhibitors and incubation conditions that cause selective inhibition of alanine aminotransferase or aspartate aminotransferase. Satisfactory results were obtained by preincubating cells with L-cycloserine or L-2-amino-4-methoxy-trans-but-3-enoic acid in the absence of added substrates. When cells were incubated for 20 min with 5...

The concentrations of pyridoxal 5'-phosphate, and the holoenzyme activities and apoenzyme contents of alanine aminotransferase and aspartate aminotransferase in plasma were determined simultaneously in healthy individuals, patients with renal insufficiency with and without chronic haemodialysis and in patients with acute myocardial infarction. Plasma pyridoxal 5'-phosphate is significantly dimi...

effects of ambient copper was investigated on serum stress markers, sodium and enzyme levels in common carp ( cyprinus carpio l.) over a 14-d exposure period. fish were exposed to 0, 25 and 100 μg l -1 copper (as copper sulfate) and blood was sampled at 0, 3, 7 and 14 d after exposure. serum profile was significantly affected by copper concentration, sampling time and their interaction. increas...

The influence of pyridoxal-5'-phosphate on the activities of alanine aminotransferase and aspartate aminotransferase from commercial test sera was investigated. Enhancement of the activity of alanine aminotransferase by the coenzyme is about 20% when 0.1 mmol/1 of pyridoxal-S'-phosphate is added to the reaction medium. This concentration seems sufficient for full saturation of the apoenzyme. Fo...

We describe the development of a sensitive, specific radioimmunoassay for the cytoplasmic and mitochondrial isoenzymes of human aspartate aminotransferase (L-aspartate:2-oxoglutarate aminotransferase; EC 2.6.1.1). Isoenzymes from human heart tissue were purified to homogeneity and used to raise high-titer antisera in rabbits. We partly purified the antisera by selective column chromatography. T...

Aspartate aminotransferase (L-aspartate: 2-oxoglutarate aminotransferase, EC 2.6.1.1, glutamic-oxalacetic transaminase, GOT) comprises two isoenzymes. One is anionic and is associated principally with the soluble fraction of the cytoplasm, the other is cationic and is associated with the mitochondria [4]. A sensitive semi quantitative assay has been developed for cationic aspartate aminotransfe...

background: this study aimed to assess several biochemical and oxidative stress parameters before and after radioiodine therapy in patients with well-differentiated thyroid cancer who consumed a low iodine diet and withdrawal of levothyroxine after total thyroidectomy. methods: we enrolled 40 candidates for radioiodine therapy. blood sampling was performed prior to as well as 72 h after consump...