Partial purification of a thioredoxin system from Novikoff ascites hepatoma cells has been previously reported (MOORE, E. C. (1967) Biochem. Biophys. Res. Commun. 29, 264-268). Thioredoxin from the same mammalian source has now been purified to electrophoretic homogeneity by ammonium sulfate fractionation, heat treatment, DEAE-cellulose chromatography, and Sephadex chromatography. 1-Dimethylami...

1. Rabbit anti-(rat foetal liver) serum, absorbed with adult rat liver cells, decreased the electrophoretic mobility of foetal liver cells by 51% and rat hepatoma cells by 45%, indicating the presence of a foetal-type antigen on the hepatoma cell membrane. 2. The chemical nature of the surface antigen was investigated. Incubation with neuraminidase had no effect on adult liver cells but decreas...

Hepatoma Research is an open access journal and focuses on all topics related to hepatoma. The following articles are especially welcome: pathogenesis, clinical examination early diagnosis of hepatoma, complications their preventions treatments, etc.

To elucidate the mechanism for hyperlipidemia in the hepatoma-bearing state, changes in some parameters related to the lipid metabolism and serum tumor necrosis factor-alpha (TNF-alpha) level were examined in Donryu rats that had been subcutaneously implanted with an ascites hepatoma cell line of AH109A. These parameters were also examined in rats that had been given a single injection of lipop...

The mitochondria isolated in isotonic sucrose from AH-130 Yoshida ascites hepatoma appear spontaneously swollen (11, 12). This spontaneous swelling may be in part responsible for the inability of tumor mitochondria to undergo extensive volume changes in hypotonie conditions or in conditions that induce large-amplitude, metabolic-dependent, or metabolic-independent swelling in normal liver mitoc...

Gilvocarcin V, isolated rom a Streptomyces culture showed activity against experimental tumors such as sarcoma 180, Ehrlich carcinoma, Meth 1 fibrosarcoma, MH134 hepatoma and lymphocytic leukemia P388. In particular, 40% of treated mice survived for 60 days, after intraperitoneal administration of gilvocarcin V to mice bearing Ehrlich ascites carcinoma. But it was marginally active against B16...

The incorporation of tritiated thymidine into DNA molecules was studied by electr.:m microscopic autoradiography. To make autoradiogram in electron microscopic level, DNA was extracted from rat ascites hepatoma (AH 130) cells after in vitro incubation with tritiated thymidine. Extracted DNA samples were rotary shadowed with platinum palladium and covered with emulsion. Silver grains demonstrate...

A simple method to detect the existence of multiple forms of deoxythymidine was established. With this method, the pattern of the enzyme was examined in various normal and tumor tissues. The existence of multiple forms of the enzyme was demonstrated in tumor tissues such as Yoshida sarcoma, Ehrlich ascites, Hepatoma AH 130, Sarcoma 180, and Morris Hepatomas 7793 and 7794A, but not in normal rat...

The incorporation of thymidine in Novikoff hepatoma cells and Ehrlich ascites cells was severely and irreversibly inhibited by the polyenic antibiotics filipin and lucensomycin, known for their action on phospholipid-cholesterol membranes. No inhibition could be shown on regenerating liver cells or on acellular preparations from tumor cells. Despite the well-known site of action of these antibi...