Abstract Problem Does aquaporin 3 (AQP3) affect the migration and invasion of human extravillous trophoblast (HTR8/Svneo) cells? Method study A lentivirus infection system was used to construct stable cell lines with either AQP3 knockdown or overexpression. RT-PCR western blotting were verify efficiencies overexpression in HTR8/Svneo cells at mRNA protein levels, respectively. Cell Counting Kit...

By using immunohistochemical techniques applied to confocal microscopy, the presence of aquaporin 3 water channel in the epidermis of Triturus italicus (Amphibia, Urodela) has been shown. We analysed the expression of aquaporin 3 (AQP3) during the larval, pre-metamorphic and adult phases; we also showed the localization of the water-channel protein AQP3 in free-swimming conditions and during ae...

To the Editor: In their review article on water balance, Knepper et al. (April 2 issue)1 discuss water channels (aquaporins) in renal tubular cells. They omit mention of the critical role played by aquaporin-1 in microvascular endothelia. In the renal microvasculature, endothelial aquaporin-1 mediates the osmotic water efflux across descending vasa recta and is required for regulation of medull...

AsIII uptake in living cells is through aquaglyceroporin transporters, but it is unknown in arsenic-hyperaccumulator Pteris vittata. We investigated the effects of AsIII analogs glycerol and antimonite (SbIII) at 0-100 mM and aquaporin inhibitor AgNO(3) at 0-0.1 mM on the uptake of 0.1 mM AsIII or AsV by P. vittata over 1-2 h. Glycerol or SbIII didn't impact AsIII or AsV uptake by P. vittata (p...

Semiquantitative immunoblotting was used to investigate the expression levels of the four major renal aquaporins, the Na-K-2Cl cotransporter of the thick ascending limb, the type 3 Na-H exchanger, and the Na-K-ATPase in kidneys from rats with cirrhosis secondary to common bile duct ligation (CBDL). These rats had significant water retention and hyponatremia. In contrast to models of cirrhosis i...