conclusions a high prevalence of multiple β-lactamase production was observed among the ampc producers (60%), of which the majority co-produced ampc and mbl. the current study results showed correlation between β-lactamase production and the presence of antibiotic resistance genes in the isolates. results all isolates were multidrug resistant. ampc, esbl and mbl production were observed in 35 (...

salmonella enterica is recognized as one of the major food-borne pathogens with more than 2,500 serotypesworldwide. the present study addresses antimicrobial resistance of salmonella enterica serovar enteritidisisolates in iran. a collection of 151 salmonella spp. isolates collected from poultry were serotyped toidentify salmonella enteritidis. sixty-one salmonella enteritidis were subsequently...

Molecular methods that enable the detection of antimicrobial resistance determinants are critical surveillance tools that are necessary to aid in curbing the spread of antibiotic resistance. In this study, we describe the use of the Antimicrobial Resistance Determinant Microarray (ARDM) that targets 239 unique genes that confer resistance to 12 classes of antimicrobial compounds, quaternary ami...

β-lactamases production represents a determining factor in bacterial resistance to β-lactam antimicrobial agents. Over 200 enzymes have been characterized for their nucleotidic sequences and/or biochemical properties [1]. Among class A β-lactamases [2] TEM-1 is the commonest enzyme in Enterobacteriaceae. Because of wide spread use of antibiotics there has been the emergence of different variant...

Background: Widespread use and misuse of anti-infective agents have resulted in problems of drug resistance linked to treatment of infectious diseases caused by worrisome pathogens such as Pseudomonas aeruginosa. Infections caused by Pseudomonas aeruginosa with resistance to extended spectrum β-lactams and the carbapenems is emerging and requires the identification of effective alternative anti...

Antimicrobial resistance is a major health problem worldwide, but marked variations in the resistance profiles of bacterial pathogens are found between countries and in different patient settings. In Tunisia, the strikingly high prevalence of resistance of bacteria to penicillins and cephalorosporins drugs including fourth generation in clinical isolates of Gram negative bacteria has been repor...

Shotgun proteomics using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was applied to detect β-lactamases in clinical Acinetobacter baumannii isolates. The correlation of the detection of β-lactamase proteins (rather than PCR detection of the corresponding genes) with the resistance phenotypes demonstrated an added value for LC-MS/MS in antimicrobial susceptibility testing.

Overuse and misuse of antibiotics leads to rapid evolution of antibiotic-resistant bacteria and antibiotic resistance genes. Klebsiella pneumoniae has become the most common pathogenic bacterium accountable for nosocomial infections due to its high virulence factor and general occurrence of resistance to most antibiotics. The β-lactamase signaling pathway has been suggested to be involved in an...

Emergence and spread of antimicrobial resistance is a major concern for the dairy industry worldwide. Objectives were to determine: (1) phenotypic and genotypic prevalence of drug-specific resistance for 25 species of non-aureus staphylococci, and (2) associations between presence of resistance determinants and antimicrobial resistance. Broth micro-dilution was used to determine resistance prof...

Escherichia albertii is an emerging member of the Enterobacteriaceae causing human and animal enteric infections. Antimicrobial resistance among enteropathogens has been reported to be increasing in the past years. The purpose of this study was to investigate antibiotic resistance and resistance genes in E. albertii isolated from Zigong city, Sichuan province, China. The susceptibility to 21 an...