Routine disc diffusion susceptibility tests (Bauer-Kirby technique), employing 5% sheep blood-Mueller-Hinton agar and 10-mug gentamicin sulfate discs, disclosed that a significant number of clinical enterococcal isolates were sensitive to the antibiotic, as also revealed by the agar dilution technique. With few exceptions, the isolates proved resistant to this antibiotic when tested for suscept...

A variety of devices such as stainless-steel or ceramic cylinders, single or multiple in design, and paper discs of several sizes are commonly used for antibiotic assays performed by the agar diffusion method. Holes cut in agar (agar wells) are wells with melted agar is often necessary to obtain uniform inhibition zones (2). This note describes a method for the preparation of agar-well plates w...

The bacteriological control of antibiotic therapy is becoming increasingly important in view of the steady production in the U.S.A. of potent new antibiotics, some of which have a limited range of activity. It is therefore necessary to have a rapid and reliable method of carrying out the sensitivity tests in order to make this a practical routine procedure. The dried disc technique gives satisf...

Plant extracts contain secondary metabolites such as flavonoids and phenolic compounds with antibiotic activity. Antibiotic activity test can be done by using the Kirby-Bauer disc method which is indicated formation of a clear zone. This research was conducted Moringa oleifera leaves Opuntia cochenillifera cladode extracted ethyl acetate solvent. against Staphylococcus aureus bacteria in Mannit...

The sensitivities to cefuroxime and cephradine of potentially pathogenic bacteria isolated in two British general hospitals comprising 900 beds have been assessed. In a three-month period 2537 strains were studied; 30 microgram cefuroxime discs were used with 2511 strains, and cephradine discs of the same strength were used with 2525 strains. The organisms were also examined routinely for sensi...

A variety of factors which might affect zone sizes were studied with strains of Corynebacterium diphtheriae; a standard disc method for antimicrobial sensitivity testing was used. Moderate variations in inoculum size, inoculum preparation, and pH of Mueller Hinton agar (MHA) did not appreciably affect zone sizes. The addition of blood to MHA was necessary to insure the growth of all C. diphther...

OBJECTIVES This study was performed to investigate the effects of different intracanal medicaments on chemical structure and microhardness of dentin. MATERIALS AND METHODS Fifty human dentin discs were obtained from intact third molars and randomly assigned into two control groups and three treatment groups. The first control group received no treatment. The second control group (no medicamen...