GCase (glucosylceramidase) from Paenibacillus sp. TS12, a family 3 glycosidase, hydrolyses the beta-glycosidic linkage of glucosylceramide with retention of anomeric configuration via a two-step, double-displacement mechanism. Two carboxyl residues are essential for catalysis, one functioning as a nucleophile and the other as a general acid/base catalyst. p-nitrophenyl beta-D-glucopyranoside [K...

Ripe fruit offer readily available nutrients for many animals, including fruit fly larvae (Diptera: Tephritidae) and their associated rot-inducing bacteria. Yet, during most of their ontogeny, fruit remain chemically defended and effectively suppress herbivores and pathogens by high levels of secondary metabolites. Olive flies (Bactrocera oleae) are uniquely able to develop in unripe olives. Un...

The recently identified phylogenetic subgroup B5 of fungal glycoside hydrolase family 18 genes encodes enzymes with mannosyl glycoprotein endo-N-acetyl-β-D-glucosaminidase (ENGase)-type activity. Intracellular ENGase activity is associated with the endoplasmic reticulum associated protein degradation pathway (ERAD) of misfolded glycoproteins, although the biological relevance in filamentous fun...

antioxidants are compounds which can prevent biological and chemical substances from oxidative damage by reactive oxygen species. flavonoids are the most important class of polyphenolic compounds that because of their antioxidant characters possess biological activities and pharmacological effects. chrysin-6-c-fucopyranoside and chrysin-3-malonyl-6-c-fucopyranoside are mono c-glycosyl derivativ...

plants firing fuel oil produce huge quantities of fly ash as solid waste, which needs to becharacterized prior to its proper management. it is worth mentioning that the published literature lackcomprehensive information on the characteristics of such fly ash. characteristics of fuel oil fly ash producedat selected power (pp) and water desalination (dp) plants were determined. the results showed...

Taxifolin glycoside is a new drug candidate for the treatment of atopic dermatitis (AD). Many drugs cause side effects such as long QT syndrome by blocking the human ether-a-go-go related gene (hERG) K(+) channels. To determine whether taxifolin glycoside would block hERG K(+) channels, we recorded hERG K(+) currents using a whole-cell patch clamp technique. We found that taxifolin glycoside di...

Key to the effectiveness of fruit fly (Diptera: Tephritidae) suppression efforts using insecticidal bait sprays is the determination of how long the bait remains attractive to adult flies after application. Using a comparative approach, field studies were conducted in commercial papaya (Carica papaya L.; Brassicales: Caricaceae) orchards in Hawaii with the goal of quantifying the response of Ba...

the identification of different species of simuliidae, also called “black fly”, is based on particular structure of oral and terminal appendages of their larvae add also on the special morphology of adults and pupaes. because of their considerable flight range, the problem of finding their resting places, and also the difficulties of collecting the adult flies, breeding of this insect, in the l...

BACKGROUND Steviol glycoside biosynthesis pathway has emerged as bifurcation from ent-kaurenoic acid, substrate of methyl erythritol phosphate pathway that also leads to gibberellin biosynthesis. However, the genetic regulation of steviol glycoside biosynthesis has not been studied. So, in present study RNA interference (RNAi) based Agrobacterium mediated transient gene silencing (AMTS) approac...

Selagin-7-O-(6''-O-acetyl-)-ß-D-glycoside, a new flavone glycoside isolated from Cancrinia discoidea, is known to exhibit anti-inflammatory activity in vivo. This study aimed to investigate the protection of this flavone glycoside on inflammation in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. The effects of selagin-7-O-(6''-O-acetyl-)-ß-D-glycoside on inflammatory cytokines and signali...